Skip to Main content Skip to Navigation
Theses

Elaboration d’une nouvelle plateforme de développement de traceurs in vivo : application à l’imagerie de la néoangiogenèse tumorale

Olivier Martinage 1
1 LCV - Laboratoire Chimie pour le Vivant, ingénierie moléculaire pour la santé
SIMoS - Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) : DRF/JOLIOT
Abstract : Molecular imaging is an essential non-invasive tool usable for diagnosis and characterisation of many diseases. Technetium-based tracers are the most popular ones due to disponibility, cost and radiochemical properties of 99mTc. Nevertheless, effective tracers development requires a long, expensive, and mainly empirical optimisation process. This context prompted us tu carry on the development of a new technetium structure which exhibits lots of potential functionalisation spots compatible with a combinatorial approach.We synthesised 12 N3X (X = N, O, S) different ligands. Each of them includes a triazole moiety, (formed via a click-chemistry reaction), which is involved in the metal complexation that implies one of its nitrogen atoms. Then we evaluated their ability to readily form oxotechnetium complexes in conditions that are compatible with medical use in hospital. One complex was formed in quantitative yields and its stability in mice plasma was investigated. A complex called TriaS-99mTc, stable to more than 90% after 6h incubation, was selected. In vivo study of TriaS-99mTc revealed an efficient blood clearance via the urinary excretion pathway with very low degradation.As an application, we used this structure for the development of tracers that target integrin αvβ3, a known biomarker of tumor neoangiogenesis. First, we synthesised functionnalised TriaS-based integrated complexes. Fonctionnal modification of TriaS by addition of side chains and substituents did not affect its ability to chelate oxotechnetium quantitatively. In addition, its stability in mice plasma was satisfactory. We also developped a bifonctionnal approach using c(RGDfK) peptide as the targeting biomolecule. In this way, a variable moiety (herein a PEG moiety) can be inserted in the structure through click-chemistry in order to modulate tracers solubility, biodistribution and excretion.
Document type :
Theses
Complete list of metadatas

Cited literature [227 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00765925
Contributor : Abes Star :  Contact
Submitted on : Monday, December 17, 2012 - 11:07:18 AM
Last modification on : Monday, October 19, 2020 - 10:58:31 AM
Long-term archiving on: : Sunday, December 18, 2016 - 2:56:44 AM

File

VD_Martinage_Olivier_08102012....
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00765925, version 1

Collections

Citation

Olivier Martinage. Elaboration d’une nouvelle plateforme de développement de traceurs in vivo : application à l’imagerie de la néoangiogenèse tumorale. Sciences agricoles. Université Paris Sud - Paris XI, 2012. Français. ⟨NNT : 2012PA114841⟩. ⟨tel-00765925⟩

Share

Metrics

Record views

969

Files downloads

713