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Modification électrochimique de surface pour la mesure des interactions ADN/Protéines (HsRad51 - Transposase)

Abstract : Since the emergence of the term "biosensor" through an article of Clark and Lyons in 1962, such devices have experienced a tremendous activity both in the academic and industries. The main objective of this thesis work was to create a surface allowing the specific immobilization of single or double DNA strand by covalent bonding and then study the interactions that may exist between a given protein and DNA. To functionalize the surface, we firstly investigated the electrochemical reduction of aryldiazoniums salt. This kind of methodology has allowed us to covalently graft Ar-SO2Cl functions over the conductive surface which can further react with DNA to immobilize it. By using the QCM and AFM methodologies, we are able to kinetically or structurally detail the intimate mechanisms of interactions between two proteins (HsRad51 and Transposase) and single or double strand DNAs.
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https://tel.archives-ouvertes.fr/tel-00752894
Contributor : ABES STAR :  Contact
Submitted on : Friday, November 16, 2012 - 3:52:14 PM
Last modification on : Tuesday, December 14, 2021 - 10:26:03 AM
Long-term archiving on: : Saturday, December 17, 2016 - 11:50:41 AM

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2012LEMA1012_converti.pdf
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  • HAL Id : tel-00752894, version 1

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Charles Esnault. Modification électrochimique de surface pour la mesure des interactions ADN/Protéines (HsRad51 - Transposase). Chimie théorique et/ou physique. Université du Maine, 2012. Français. ⟨NNT : 2012LEMA1012⟩. ⟨tel-00752894⟩

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