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Theses

Fidélité de la traduction chez les eucaryotes. De la molécule au génome

Abstract : This report describes a study of translation fidelity in Eukarya. Two aspects are tested: the molecular mechanism of recoding and the research of recoding events at the genomic level. During my PhD I have developed three projects:The first project deals with the role of the elongation factor eEF2 in reading frame maintenance. The strategy is based on a random mutagenesis of EFT2 and a phenotypic screening in order to isolate mutants increasing or reducing -1 frameshifting efficiency.The second project describes the development of a single molecule translational system to study the eukaryotic ribosome. Translation initiation is mediated by the CrPV IRES which is initiation factor and initiation tRNA independent. Elongation is monitored with the dissociation of a fluorescent oligonucleotide by the helicase activity of the ribosome. This work is a proof of principle that studying eukaryotic ribosome with single molecule techniques is now feasible.The third project is a comparative genomic approach to search for recoding and unconventional translational events in the genome of yeast Saccharomyces cerevisiae. The approach is based on the detection of conserved genomic organization among 19 Fungi genomes. The candidate genes are then tested in vivo with a dual reporter system. This study allowed the characterization of VOA1 which was further analysed.
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https://tel.archives-ouvertes.fr/tel-00749760
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Submitted on : Thursday, November 8, 2012 - 12:47:19 PM
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Hélène Chommy. Fidélité de la traduction chez les eucaryotes. De la molécule au génome. Sciences agricoles. Université Paris Sud - Paris XI, 2012. Français. ⟨NNT : 2012PA112193⟩. ⟨tel-00749760⟩

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