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Développement de stratégies d'imagerie multimodalités pour la pharmacologie des agents anticancéreux

Abstract : Preclinical imaging in oncology is booming. It allows, using representative animal models of human cancers, to understand the mechanisms of development of pathologies and to assess the therapeutic efficiency of a new treatment. The main objective of this work was to develop two orthotopic models of cancer (pancreas and colon) and to assess on them the reference treatments as well as a new therapeutic strategy by non thermal plasma so called Plasma Gun. The two cancer models developed showed good representation in relation to human cancers, with the appearance of distant metastases and hypoxia. 5-fluorouracil for the HCT116-luc orthotopic model of colorectal carcinoma and gemcitabine for the MIA PaCa2-luc pancreatic adenocarcinoma model, have induced discrete effects at low dose wich can be detected thanks imaging modalities. After validation of our experimental steps, a new therapeutic strategy, Plasma Gun was evaluated and showed significant effects on tumor growth inhibition. The second objective was to carry out tools for the induction and the characterization of bone metastases and for high resolution imaging of the vasculature. On the one hand, bone metastases obtained by injection of PC3M-luc cells intracardially, was evaluated and quantified with different imaging modalities (bioluminescence, scintigraphy and Computed Tomography). And the other hand, the achievement of a high resolution imaging of vascularization, was possible by the casting method that restores the 3D structure of the vascular architecture following injection of a resin in the circulation.
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https://tel.archives-ouvertes.fr/tel-00747343
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Submitted on : Wednesday, October 31, 2012 - 10:02:20 AM
Last modification on : Wednesday, May 4, 2022 - 10:17:41 AM
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Laura Brullé. Développement de stratégies d'imagerie multimodalités pour la pharmacologie des agents anticancéreux. Médecine humaine et pathologie. Université d'Orléans, 2012. Français. ⟨NNT : 2012ORLE2015⟩. ⟨tel-00747343⟩

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