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Regulation de la nadph oxydase phagocytaire par la pat1 « protein interacting with app tail 1

Abstract : Reactive oxygen species (ROS) production by the phagocyte NADPH oxidase plays a crucial role in host defenses. NADPH oxidase is composed of the membrane flavocytochrome b558 components (p22phox and gp91phox/NOX2), and cytosolic components (p40phox, p47phox, p67phox and a small GTPase Rac1 or Rac2). In this work we identified PAT1 by double hybrid system as a potential partner of p22phox. The interaction between p22phox and PAT1a was further confirmed by in vitro GST pull-down assay, confocal microscopy and co-immunoprecipitation. Addition of recombinant PAT1a to the cell free-system enhanced NADPH oxidase activation and it’s over-expression in human monocytes and in COSphox cells increased ROS production in resting and fMLP-stimulated cells.These data clearly identify PAT1 as a novel regulator of NADPH oxidase activation in phagocytes.Inhibition of p22phox/PAT1 interaction could be used as new approach to limit ROS production by phagocytes at inflammatory sites.
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https://tel.archives-ouvertes.fr/tel-00739376
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Submitted on : Monday, October 8, 2012 - 10:18:13 AM
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Riad Arabi Derkawi. Regulation de la nadph oxydase phagocytaire par la pat1 « protein interacting with app tail 1. Immunologie. Université Paris Sud - Paris XI, 2011. Français. ⟨NNT : 2011PA11T061⟩. ⟨tel-00739376⟩

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