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Contribution à l'élaboration d'une plateforme miniaturisée de test en routine du pouvoir infectieux d'agents pathogènes: application à Cryptosporidium sp.

Abstract : This work focuses on the development of a microfluidic platform (lab on a chip) for the measurement of pathogens infectivity power in water. The main function studied in this manuscript is the concentration of parasites suspended in a liquid. The manipulation of microparticles is based on the use of electrokinetic forces. Numerical analysis in two and three dimensions provides qualitative information. It can also extract the geometric key values of electrodes used for the concentration of the microparticles. These theoretical results are confirmed experimentally using two devices containing a wide variety of concentrators. From the theoretical and experimental results, we design an optimized concentrator. It is integrated in a system allowing droplet motion by electrowetting on dielectric (EWOD). This system is used in three modes: concentrator, extractor and separator. Cryptosporidium oocysts and Giardia lamblia cysts are used for the characterization of the device. Preliminary results of cell culture on surfaces functionalized at scale of hundreds of microns allow to consider the development of a complete microfluidic platform for infectivity analysis of pathogenic agent such as Cryptosporidium.
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https://tel.archives-ouvertes.fr/tel-00738714
Contributor : Romuald-Alexis Lejard-Malki <>
Submitted on : Thursday, October 4, 2012 - 11:56:36 PM
Last modification on : Wednesday, February 17, 2021 - 10:58:08 AM
Long-term archiving on: : Saturday, January 5, 2013 - 4:00:33 AM

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  • HAL Id : tel-00738714, version 1

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Romuald-Alexis Lejard-Malki. Contribution à l'élaboration d'une plateforme miniaturisée de test en routine du pouvoir infectieux d'agents pathogènes: application à Cryptosporidium sp.. Micro et nanotechnologies/Microélectronique. Université des Sciences et Technologie de Lille - Lille I, 2012. Français. ⟨tel-00738714⟩

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