S. botryosum subsp. vesuvianum (Pers.)Ach. ; S. alpinum var. vesuvianum (Pers.)Link ; S. denudatum var. vesuvianum (Pers.)Laur. ex, Hepp. ; S. denudatum Flörke S. graminosum Schaer. S. turgescens Nyl ,
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4 g) est purifiée par une chromatographie échangeuse d'ions sur gel de Dowex 50W-X8 en utilisant le protocole suivant: 250 g de résine sulfonate échangeuse de cations : DOWEX 50W-X8 (copolymère styrène + divinylbenzène, sur lequel sont greffés des groupes -SO 3 H) sont pesés, lavés abondamment ,
) et une fraction de 1500 ml est d'eau est ajoutée pour l'élution ,
NaCl se retrouvant dans la fraction purifiée, le résidu sec est repris dans 200 ml d'EtOH anhydre. Le NaCl non soluble dans l'EtOH est alors éliminé, en reprenant le surnageant. Celui-ci est alors évaporé à sec au rotavapor (= fraction Aq-III (2,50 g)). La dernière, colonne éluée avec du méthanol et la solution obtenue a été évaporée ,
La fraction II-0 (435 mg) est chromatographiée sur résine échangeuses d'ions, DOWEX 50W-X8 (45 g) en utilisant le même protocole que précédemment, puis purifié par HPLC préparative (colonne C18 avec comme phase mobile de ,
50 g) a été séparée par chromatographie flash sur gel de RP-C18 (systéme de solvant de H2O-MeOH, 100:0 à 0:100) pour obtenir 88 fractions de 10 ,
Après réunion des fractions ayant un méme profil CCM, nous obtenons 5 fractions (III FC A-E) et ensuite fraction III FC B (525 mg) a été purifiée par HPLC préparative avec une colonne C18 en utilisant H2O, MeOH et le composant Sma3 (8 mg) est obtenu, p.26 ,
MHz, acetone-d 6 ) C-2 ,
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sticta Physciaceae/physcia et pyxine Pannariaceae /pannaria Coccocarpiaceae /coccocarpia Lecanotaceae /lecanora Pertusariaceae /pertusaria Lecideaceae /lecidea Graphidaceae/graphis Roccellaceae /opegrapha et chiodecton Melaspileaceae /melaspilea Graphidaceae/glyphis Verrucariaceae/verrucaria Trypetheliaceae/trypethelium L'ouest Java, 1866. ,
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