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Authentification de l'alimentation des ruminants par analyse des composés volatils de leur tissus adipeux

Abstract : There is an increasing consumer demand for robust analytical tools for objective authentication of the processing practices in food production chains, in particular for animal products. The aim of this work was to develop an authentication tool of grass feeding in lambs by analysing the volatile fraction of their adipose tissues. The animals were raised under experimental conditions allowing a strict control of feeding. The perirenal, caudal sub-cutaneous, an heart fat tissues were analysed by Dynamic Headspace - Gas Chromatography - Mass Spectrometry (DH-GC-MS). The analytical signal was stabilized and the drifts were corrected by using chemometrics tools. The purpose of the first step of the study was to identify the compounds discriminating the adipose tissues of lambs fed either pasture or concentrate. Using an improved analytical procedure, 126 pasture diet tracers were identified. This experiment also showed the interest of analyzing different types of adipose tissues in parallel for discriminating the two diets. The second step of the study aimed at assessing the relevance of the volatile fraction of lamb adipose tissues to authenticate alternate diets. These diets, widely used for the ruminant rearing, consist in switching from pasture to a concentrate based diet before slaughter, or vice versa. The variations in the amounts of pasture diet volatile tracers were monitored in different adipose tissues of lambs fed with such alternate diets. Volatile compounds enabled to discriminate lambs fed at pasture then finished on a concentrate diet for periods corresponding to 0, 4, 8, or 12 kg of the final weight gain. Likewise, the lambs fed concentrate and finished at pasture for periods corresponding to 0, 17, 51, 85 days were successfully discriminated. In addition to litterature data, all the experimental rearing systems used in this work allowed to reveal 19 particularely robust and generic volatile tracers of pasture diet. This work evidenced the feasibility of the authentication of feeding practices of lambs fed alternate diets. It also reinforces the ability of procedures of analytical signal treatment like instrumental drifts correction and ratios of variables to improved the discrimination power of authentication models.
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Submitted on : Wednesday, September 12, 2012 - 2:43:23 PM
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  • HAL Id : tel-00731275, version 1
  • PRODINRA : 244510



Guilhem Sivadier. Authentification de l'alimentation des ruminants par analyse des composés volatils de leur tissus adipeux. Alimentation et Nutrition. Université Blaise Pascal - Clermont-Ferrand II, 2008. Français. ⟨NNT : 2008CLF21890⟩. ⟨tel-00731275⟩



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