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Suivi in vivo et en temps réel du processus infectieux induit par Yersinia pestis

Toan Nham 1
Abstract : The enterobacteria Yersinia pestis causes plague, a deadly infectious disease infamously known for millions of death caused during three major pandemics. The bacterium possesses virulence mechanisms that have not been completely elucidated. Mouse model has long been used for pathology studies, yet usual experimentation is time-consuming and laborious and requires large groups of animals. Bioluminescence imaging provides an efficient improvement in infection monitoring by providing non-invasive, real-time in vivo detection of living bacteria. We applied this technique to Y. pestis virulent strain CO92 and proved that this bacterium could emit detectable bioluminescence signals both in vitro and in vivo. Light intensity was correlated to cfu charges in spleen, liver and inguinal lymph node. By monitoring bubonic plague, we observed the sequence of bacterial colonization steps, from the injection site to the inguinal draining lymph node. The next target was the ipsilateral axillary lymph node: we proved that bacteria followed a direct path from the inguinal lymph node to the axillary lymph node. The bacteria then reached and colonized blood-filtering organs such as the spleen and the liver. In the latest times of the disease, septicemia was observed with a typical whole-body bioluminescence. Our results showed that the high variability in the kinetics of bubonic plague was attributable to the length of time during which Y. pestis remained confined in the injection site. Once the bacteria had reached the draining lymph nodes, the disease evolved in a very fast and regular way to septicemia within two days.
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Submitted on : Wednesday, September 12, 2012 - 11:42:26 AM
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Toan Nham. Suivi in vivo et en temps réel du processus infectieux induit par Yersinia pestis. Bactériologie. Université Paris-Diderot - Paris VII, 2012. Français. ⟨tel-00731170⟩

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