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Cultivo de células osteoprogenitoras em compósito 3-D hidroxiapatita-colágeno sob condições estática e dinâmica

Abstract : The progress in Tissue Engineering area allows the development of biomaterials that mimic the properties of natural tissues. For biomedical applications in mineralized tissues, composites based on hydroxyapatite (HA) and collagen (Col) have presented good results when implanted in vivo. The aim of this work was to produce a 3-D matrix and to observe the cell behaviour when stromal cells are cultured in contact with HA-Col scaffold under static and dynamic conditions. For in vitro biological evaluation, osteoprogenitor human cells (Stro+1A cells) were grown and their colonization capacity and adhesion, proliferation and differentiation behaviour were quantified. Two perfusion flow rates (0,03ml/min and 0,3ml/min) were proposed for dynamic culture. The HA-Col composite was prepared by reorganization of Col fibrils simultaneously with HA crystal nucleation and precipitation from calcium and phosphate rich solutions. Afterwards, the composites were crosslinked and sterilized by gamma radiation. Stro+1A cells were inoculated (5x105 cells/sample) into the scaffolds and cultured over 21 days in a humid incubator at 37°C and 5% CO2. Infrared spectroscopy and X-ray diffraction results suggested a calcium-deficient hydroxyapatite as mineral phase. About cell culture, the cell number increased under higher flow rate dynamic culture. By scanning electron microscopy and histological sections, we observed cells adhered and spread inside colonized scaffolds.
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Submitted on : Tuesday, August 28, 2012 - 3:12:10 PM
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Doris Moura Campos. Cultivo de células osteoprogenitoras em compósito 3-D hidroxiapatita-colágeno sob condições estática e dinâmica. Agricultural sciences. Université de Haute Alsace - Mulhouse; Institut Alberto Luiz Coimbra d'études doctorales et de recherche en ingénierie (Rio de Janeiro), 2012. Portuguese. ⟨NNT : 2012MULH6331⟩. ⟨tel-00725990⟩

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