Skip to Main content Skip to Navigation
Theses

Avaliação in vitro do comportamento de células osteoprogenitoras e macrófagos humanos em pastilhas de fosfato tricálcico com e sem magnésio

Abstract : Research on bone tissue regeneration is constantly expanding due to the improvement of life expectancy with a consequent increase in the rate of chronic diseases. The aim of this work was to evaluate the in vitro behavior of STRO+1A osteoprogenitor cells cultured onto dense tablets of tricalcium phosphate doped (β-TCMP, Mg/Ca = 0.15) or not (β-TCP) with magnesium under static and dynamic (flow rate of 0.3 mL/min), as well as the response of human macrophages after contact with the granules extracts of the materials (ISO10993-12: 2007). The β-TCMP and β-TCP tablets were obtained by sintering a calcium-deficient apatite doped with magnesium and commercial TCP (Merck), respectively. The diffractograms and infrared spectra confirmed the presence of β-TCP and β-TCMP with a Mg/Ca ratio of 0.14 (plasma spectrometry). A cell density of 5x103 was inoculated onto the tablets and then STRO+1A were cultured at 37°C/5% CO2 for up to 21 days, in which the proliferation and differentiation rate were assessed. There was no difference in cell density between the materials after 21 days of culture, neither under static nor dynamic conditions; however, it seems that the dynamic environment accelerated osteoblast differentiation. In a general way, β-TCMP did not change the response of macrophages (cytokine profile), activated or not, by bacterial lipopolysaccharide, cultured for up to 72 hours with the extracts of biomaterials, when compared to β-TCP. Based on these results, both materials appear to be adequate for bone loss therapy.
Document type :
Theses
Complete list of metadata

Cited literature [17 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00720079
Contributor : Abes Star :  Contact
Submitted on : Monday, July 23, 2012 - 3:17:10 PM
Last modification on : Wednesday, November 13, 2019 - 12:06:25 PM
Long-term archiving on: : Wednesday, October 24, 2012 - 2:45:29 AM

File

2012MULH6651_these_DOSSANTOSTA...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00720079, version 1

Collections

Citation

Débora dos Santos Tavares. Avaliação in vitro do comportamento de células osteoprogenitoras e macrófagos humanos em pastilhas de fosfato tricálcico com e sem magnésio. Other. Université de Haute Alsace - Mulhouse; Universidade federal do Rio de Janeiro, 2012. Portuguese. ⟨NNT : 2012MULH6651⟩. ⟨tel-00720079⟩

Share

Metrics

Record views

446

Files downloads

1458