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Facteurs génétiques impliqués dans la différenciation de l'endoderme primitif au cours du développement préimplantatoire chez la souris

Abstract : Numerous studies on the mouse embryo have allowed to caracterize the morphological events occuring at early development. Our groups aims to clarify the mechanisms of preimplantation embryogenesis in the mouse, focusing on the differentiation of the primitive endoderm (PrE). The PrE is the second extraembryonic tissue formed before implantation and required for both survival and correct development of the embryo. In order to get a better understanding of how PrE fate is established and the epitelium formed, I focused my study on two signaling pathways : retinoic acid signaling and the Wnt/β-catenin pathway. I analysed the expression of PrE and epiblast markers on a RA-driven F9 cell differentiation by quantitative RT-PCR. I show that PrE-genes are sequencially induced and in the same order as observed in vivo by immunostaining. I also adapted this protocol for preimplantation embryos. By in situ hybridations and immunostainings I characterized the expression profils of different members of the two signaling pathways.
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https://tel.archives-ouvertes.fr/tel-00719625
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Submitted on : Friday, July 20, 2012 - 12:29:56 PM
Last modification on : Tuesday, February 23, 2021 - 11:34:02 AM
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  • HAL Id : tel-00719625, version 1

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Nina Wustrack. Facteurs génétiques impliqués dans la différenciation de l'endoderme primitif au cours du développement préimplantatoire chez la souris. Génétique animale. Université Blaise Pascal - Clermont-Ferrand II; Université d'Auvergne - Clermont-Ferrand I, 2010. Français. ⟨NNT : 2010CLF1MM27⟩. ⟨tel-00719625⟩

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