Caractérisation des communautés procaryotiques impliquées dans la bioremédiation d'un sol pollué par des hydrocarbures et développement d'outils d'analyse à haut débit

Abstract : Environmental ecosystems are sensitive to damage from human activities and there is an increasing need to develop better methods for removing pollutants from soils. The removal of pollutants or their transformation to less toxic products by bioremediation is a less invasive and expensive process than classical decontamination. However, the use and optimization of bioremediation treatments requires knowledge on the microbial communities directly and indirectly involved in the degradation of the pollutants. Therefore, after the creation of a synthesis describing the microbial diversity and the available tools for the isolation and the identification of microorganisms, we have studied the structure and the dynamic of the bacterial communities during the bioremediation of an aliphatic hydrocarbon-polluted soil. Beyond the characterization of a bacterial "core-set" present throughout the decontamination process, we have identified several phylotypes potentially involved in degradation. In order to globally monitor these bacterial communities, we also have developed a probe design software (PhylArray) generating efficient and explorative probes for high-throughput molecular tools: the DNA microarrays.
keyword : Procaryotes biopuces
Complete list of metadatas

Cited literature [239 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00718551
Contributor : Camille Meyer <>
Submitted on : Tuesday, July 17, 2012 - 3:12:22 PM
Last modification on : Monday, March 11, 2019 - 9:10:04 PM
Long-term archiving on: Friday, December 16, 2016 - 12:21:04 AM

Identifiers

  • HAL Id : tel-00718551, version 1

Collections

Citation

Cécile Militon. Caractérisation des communautés procaryotiques impliquées dans la bioremédiation d'un sol pollué par des hydrocarbures et développement d'outils d'analyse à haut débit. Protistologie. Université Blaise Pascal - Clermont-Ferrand II; Université d'Auvergne - Clermont-Ferrand I, 2007. Français. ⟨NNT : 2007CLF21810⟩. ⟨tel-00718551⟩

Share

Metrics

Record views

781

Files downloads

4215