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Staphylococcus xylosus : cartographie du génome et diversité génétique

Abstract : Staphylococcus xylosus is a ubiquitous bacterium, and a commensal of the human and animals' cutaneo-mucosa. It is one of the principal starters used in fermented meat industry. Its technological properties were well-known but few genetic data on this species were available. We established the first physical and genetic map of the S. xylosus C2a strain. This map allowed estimating the chromosome size (2.89 Mb) and gave first indication of the S. xylosus chromosome organization. Within this species we showed that there was a great diversity, at once phenotypic (sugar metabolism, giant colony formation) and genotypic (genomic profiles, size of the chromosome). Following subtractive hybridization, we studied the genetic content diversity of strains from various origins. The genome of the S. xylosus C2a strain was subtracted from those of a starter strain and two strains isolated from opportunist infections. A total of 78 kb of DNA sequences were identified, the majority corresponding to metabolic genes. The distribution of strain-specific fragments within the S. xylosus species revealed two clusters. One was composed of the potentially hazardous strains. These fragments could be used in epidemiologic studies. A majority of strainspecific fragments was colocalized on the oriC region of S. xylosus chromosomes. This region corresponded to an insertion loop which allowed the acquisition of important genetic material necessary for S. xylosus fitness to various ecological niches. Sequencing this area in various strains should provide a better understanding of the S. xylosus genome plasticity.
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  • HAL Id : tel-00718141, version 1
  • PRODINRA : 244842

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Emilie Dordet-Frisoni. Staphylococcus xylosus : cartographie du génome et diversité génétique. Biochimie, Biologie Moléculaire. Université Blaise Pascal - Clermont-Ferrand II; Université d'Auvergne - Clermont-Ferrand I, 2007. Français. ⟨NNT : 2007CLF21760⟩. ⟨tel-00718141⟩

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