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Listeria monocytogenes : Caractérisation fonctionnelle d'un mutant ferritine. Etude de la biodiversité par une approche protéomique

Abstract : Listeria monocytogenes is the etiologic agent of listeriosis a foodborne infection rare but with a case fatality rate of 25% for humans. We have first characterized a ferritin mutant and shown the importance of this gene product in virulence, resistance to conditions of iron starvation and thermal or oxydative stresses. Then, we studied the biodiversity of L. monocytogenes, with a proteomic approach, by studying 12 strains of different origins, serovars and levels of virulence. Based on the intracellular and secreted protein patterns, a hierarchical clustering showed two distinct clusters, one including strains of serovar 1/2a, the other one strains of serovar 4b and 1/2b. Some protein spots either strain- or serovar-specifics were identified by MALDI-TOF mass spectrometry with the objective to better evaluate and manage the risk due to L. monocytogenes.
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https://tel.archives-ouvertes.fr/tel-00718111
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Submitted on : Monday, July 16, 2012 - 11:24:19 AM
Last modification on : Friday, October 23, 2020 - 4:49:24 PM

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  • HAL Id : tel-00718111, version 1
  • PRODINRA : 244841

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Emilie Dumas. Listeria monocytogenes : Caractérisation fonctionnelle d'un mutant ferritine. Etude de la biodiversité par une approche protéomique. Ingénierie des aliments. Université Blaise Pascal - Clermont-Ferrand II; Université d'Auvergne - Clermont-Ferrand I, 2007. Français. ⟨NNT : 2007CLF21758⟩. ⟨tel-00718111⟩

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