Particle diffusion in protein gels and at interfaces

Abstract : The objective of the thesis was to investigate the mobility of tracer particles in complex media byConfocal Laser Scanning Microscopy (CLSM) combined with multiple particle tracking (MPT)and fluorescence recovery after photobleaching (FRAP).First, we investigated the diffusion of tracer particles in gels formed by globular proteins. Gelswith a variety of structures were prepared by varying the protein and salt concentrations. Thestructure was characterized by analysis of the CLSM images in terms of the pair correlationfunction. The mobility of particles with a broad range of sizes (2nm - 1μm) was investigatedboth in homogeneous and heterogeneous gels and related to the gel structure.Second, we studied water-in-water-emulsions prepared by mixing aqueous solutions of PEO anddextran. It is shown that when colloidal particles are added they become trapped at the waterwaterinterface because they reduce the interfacial tension. The structure and the displacement ofthe particles at the interface were determined using CLSM combined with MPT.
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Theses
Other. Université du Maine, 2012. English. <NNT : 2012LEMA1002>


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Gireeshkumar Balakrishnan Nair. Particle diffusion in protein gels and at interfaces. Other. Université du Maine, 2012. English. <NNT : 2012LEMA1002>. <tel-00710630>

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