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La différenciation sporale chez les microsporidies : imagerie 3D et isolement des stades de développement, analyse de l'expression différentielle de protéines structurales et première identification des glycanes

Abstract : The microsporidian, Encephalitozoon cuniculi, an intracellular parasite, is an opportunistic pathogen. Three-dimensional reconstructions from serial sections allowed visualization of the different cell stages in sporogenesis. The immunolocalization of cell wall proteins coupled with in situ hybridization of the corresponding mRNAs revealed their differential expression during intracellular development. The study of protein glycosylation has demonstrated the absence of N-glycosylation and the existence of a path of O-mannosylation. Similar to those of fungi, are linear chains with a maximum length of eight mannoses linked alpha1, 2 and mannoproteins are localized in the polar cap. Fucosylated proteins are present in the spore wall. The development of a protocol of separation stages sporogoniques density gradient, offers opportunities for comparative biochemical analysis.
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https://tel.archives-ouvertes.fr/tel-00702783
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Submitted on : Thursday, May 31, 2012 - 12:13:22 PM
Last modification on : Tuesday, April 20, 2021 - 11:54:02 AM
Long-term archiving on: : Thursday, December 15, 2016 - 9:46:38 AM

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  • HAL Id : tel-00702783, version 1

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Vanessa Taupin. La différenciation sporale chez les microsporidies : imagerie 3D et isolement des stades de développement, analyse de l'expression différentielle de protéines structurales et première identification des glycanes. Génétique. Université Blaise Pascal - Clermont-Ferrand II, 2006. Français. ⟨NNT : 2006CLF21679⟩. ⟨tel-00702783⟩

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