Skip to Main content Skip to Navigation
Theses

Etude de la maurocalcine comme peptide de pénétration cellulaire

Abstract : Maurocalcine (MCa) is a 33 mer toxin initially identified from a tunisian scorpion venom, scorpio maurus palmatus. This peptide initially triggered our interest for its pharmacological activity on Ryanodine Receptor type 1 (RyR1) of skeletal muscles. In studying how this toxin reaches the intracellular RyR1, it has been shown that MCa could be placed in the growing family of cell penetrating peptides. Since the discovery that MCa can act as a transport agent for the intracellular delivery of fluorescent streptavidine, data have accumulated to illustrate the amazing biotechnological properties of this toxin. Several new analogs have been produced that keep cell penetration properties and lose pharmacological activity of the native molecule. This is the case for a linear analog of MCa synthesized by replacing internal cysteine residues by aminobutyric acid, or by the synthesis of a MCa analog with all its amino acid in D conformation. MCa proved efficient for the intracellular delivery of nanoparticles leading to a myriad of hi-tech applications. Finally, MCa has been grafted on an anti-tumor agent, doxorubicin, to made chemo-resistant tumor cells chemo-sensitive. So it seems that MCa begins its career as a biotechnological tool, and that this toxin will be helpful to see the light on the mechanistic aspects of RyR function.
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00685113
Contributor : Abes Star :  Contact
Submitted on : Wednesday, April 4, 2012 - 10:34:25 AM
Last modification on : Friday, November 6, 2020 - 3:49:00 AM
Long-term archiving on: : Thursday, July 5, 2012 - 2:29:16 AM

File

Poillot_Cathy_2011_archivage.p...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00685113, version 1

Collections

STAR | U836 | UGA

Citation

Cathy Poillot. Etude de la maurocalcine comme peptide de pénétration cellulaire. Autre [q-bio.OT]. Université de Grenoble, 2011. Français. ⟨NNT : 2011GRENV028⟩. ⟨tel-00685113⟩

Share

Metrics

Record views

826

Files downloads

1162