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FLUCTUATIONS DEMOGRAPHIQUES AU COURS DU CYCLE DE VIE DU CaMV (Cauliflower mosaic virus). Estimation de la taille efficace des populations virales lors de la colonisation des feuilles de la plante hôte, évaluation de la multiplicité d'infection cellulaire au sein de ces feuilles, et estimation de la taille des goulots d'étranglement lors de sa transmission d'hôte à hôte par vecteur

Abstract : Cauliflower mosaic virus (CaMV), a double-strand DNA plant virus, is non-circulatively transmitted by aphid vectors. As for many other viruses, ample demographic fluctuations along the life cycle are poorly defined, though they may have considerable impact on their evolution. In order to monitor CaMV populations we have constructed 6 infectious clones, each containing a distinct genetic marker, and developed a new analysis method: Quantitative Single-letter Sequencing (QSS) allowing to quantify their relative frequency in infected plants and/or leaves. Through the evolution of markers' frequency, we could calculate the effective size of CaMV populations: hundreds to thousands of genomes are founding the population in every single new leaf during systemic infection. This evaluation is 10 to 100 fold higher than that previously published for all other plant viruses studied. Furthermore, we have demonstrated that the multiplicity of infection of host cells (MOI) by CaMV genomes is not constant, and increases during CaMV infection to reach a maximum value around 7, largely exceeding the rare other estimates available on animal viruses or phages. Finally, using the EPG technique, we have controlled the feeding behaviour of aphids vectors when acquiring CaMV, and evaluated the impact of this vector behaviour on the size of the bottleneck induced on viral population during plant-to-plant transmission.
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https://tel.archives-ouvertes.fr/tel-00681500
Contributor : Baptiste Monsion <>
Submitted on : Wednesday, March 21, 2012 - 4:17:40 PM
Last modification on : Friday, October 23, 2020 - 4:47:14 PM
Long-term archiving on: : Friday, June 22, 2012 - 2:26:52 AM

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  • HAL Id : tel-00681500, version 1

Citation

Baptiste Monsion. FLUCTUATIONS DEMOGRAPHIQUES AU COURS DU CYCLE DE VIE DU CaMV (Cauliflower mosaic virus). Estimation de la taille efficace des populations virales lors de la colonisation des feuilles de la plante hôte, évaluation de la multiplicité d'infection cellulaire au sein de ces feuilles, et estimation de la taille des goulots d'étranglement lors de sa transmission d'hôte à hôte par vecteur. Génétique des populations [q-bio.PE]. Université Montpellier II - Sciences et Techniques du Languedoc, 2008. Français. ⟨tel-00681500⟩

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