Maturation de sites métalliques de protéines par les protéines à radical S-Adénosyl-L-méthionine et la machinerie de fabrication des centres fer-soufre

Elodie Marinoni 1
1 Institut de Biologie Structurale
IBS - UMR 5075 - Institut de biologie structurale
Abstract : FeS clusters are widely used protein cofactors, found both in bacteria and eukaryotes. They play key roles such as electron transfer, substrate binding and activation, regulation of gene expression, sulfur donor etc. They are really various, ranging from the [2Fe-2S] cluster to the more complex MoFe7S9X (X = C, N or O) agregate of nitrogenase. Clusters assembly is carried out by protein machineries. We studied the ISC (Iron-Sulfur Cluster) in bacteria, who assembles [2Fe-2S] and [4Fe-4S] clusters. It is composed of IscS, IscU, IscA, HscA, HscB proteins and a ferredoxin. Two of these proteins: the cysteine desulfurase IscS, and the scaffold protein IscU, represent the core of the machinery as IscS provides sulfur protein on IscU, which, with iron obtained from another protein (not clearly identified to date), assemble the iron-sulfur center. The latter transfers it to an apoprotein. We isolated under anaerobic conditions a stable (IscS-D35A-IscU)2 complex containing a [2Fe-2S] cluster. Different forms of the complex were obtained and their structures were solved by molecular replacement. These structures allowed us to propose a mechanism for the assembly of the [2Fe-2S] clusters at the atomic and electronic levels. We have also studied the HmdB protein, which is proposed to maturate the [Fe]-hydrogenase. HmdB is a member of the radical SAM proteins superfamily. Crystals of the apoprotein were obtained and its structure was solved by molecular replacement. Although part of the structure is not visible due to the absence of the [4Fe-4S] cluster, this structure gives a first view of the active site of the protein.
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Agricultural sciences. Université de Grenoble, 2011. French. <NNT : 2011GRENV064>


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Elodie Marinoni. Maturation de sites métalliques de protéines par les protéines à radical S-Adénosyl-L-méthionine et la machinerie de fabrication des centres fer-soufre. Agricultural sciences. Université de Grenoble, 2011. French. <NNT : 2011GRENV064>. <tel-00680023>

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