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Etude multicentrique de nouveaux marqueurs tumoraux moléculaires dans les épanchements péritonéaux et le sang : analyse par PCR quantitative en temps réel

Abstract : The natural progression of tumors is a local extension, and remotely (metastasis) by migrating cells in the blood and the lymph to secondary sites. It is therefore essential to detect circulating tumor cells in addition to morphological analysis and immunocytochemistry. In addition, two technologies (flow cytometry and RT-PCR in real time) are suitable for a rapid and sensitive automated analysis of a very small quantity of cells. The aim of our work was to develop detection systems for identification of cancer cells in peritoneal effusions and blood. The study of molecular biomarkers appears as an attractive complementary approach to improve the efficiency of diagnosis in this type of biological samples. We are interested in the identification of new tumor markers that can be used for early diagnosis and prognosis of cancer using new techniques of molecular biology. It is likely that the use of multiple molecular markers can help to raise some specific types ofcancers. We were able to develop a quantitative PCR technique in real time, significantly more sensitive than conventional cytology, and we applied this technique to the study of tumor markers in effusions, but also in blood for detecting messenger RNA. Our results show that flow cytometry well-adapted to cell lines, is not unusable for clinical specimens. For quantitative PCR, it was possible to quantify the expression levels of tumor markers using reference plasmids prepared for each gene. Several markers can differentiate malignant and benign effusions, but especially CLDN4 and Ep-CAM antigens were significantly higher (68% and 57% respectively) in patients with malignant effusions.The circulating CLDN4 mRNA was detectable and significantly higher in the sera of patients with breast cancer (64% p <0.05). The results indicate that using a combination of markers including claudin 4 is more likely to detect malignant cells and be useful for monitoring patients
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https://tel.archives-ouvertes.fr/tel-00672144
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Submitted on : Monday, February 20, 2012 - 4:03:12 PM
Last modification on : Wednesday, November 20, 2019 - 3:02:13 AM
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Fauzia Mohamed. Etude multicentrique de nouveaux marqueurs tumoraux moléculaires dans les épanchements péritonéaux et le sang : analyse par PCR quantitative en temps réel. Médecine humaine et pathologie. Université Jean Monnet - Saint-Etienne, 2010. Français. ⟨NNT : 2010STET002T⟩. ⟨tel-00672144⟩

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