Recherche bioguidée de molécules anticancéreuses issues de microalgues marines

Abstract : Marine organisms, including microalgae, constitute a major field of investigation for the discovery of new anticancer drugs. To obtain new anticancer compounds, we used a bio-guided methodology on microalgae extracts showing antiproliferative activity. This work led to the isolation of violaxanthin, an epoxycarotenoid pigment, and to the demonstration of its antiproliferative, cytotoxic, pro-apoptotic and pro-necrotic activity. Carotenoids such as fucoxanthin are already known to have anticancer activity related to the epoxide. However, this is the first study demonstrating the potential of violaxanthin.In order to isolate other pigments with anticancer activity , efficient HPLC methods were developed for their analysis and purification. Then, conventional methods of pigments extraction from microalgae were compared to innovative methods of extraction. This study demonstrates the interest of microwave technology in terms of efficiency and rapidity for the pigments extraction from frustulated microalgae (diatoms).To conclude, this work confirms the potential of marine microalgae as a source of anticancer drugs, and demonstrates the interest of microwave technology for the extraction of biologically active molecules from natural products.
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Virginie Pasquet. Recherche bioguidée de molécules anticancéreuses issues de microalgues marines. Sciences agricoles. Université de La Rochelle, 2011. Français. ⟨NNT : 2011LAROS321⟩. ⟨tel-00665228⟩

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