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Etude et valorisation des principes actifs de biominéralisation du calcium pour les applications pharmaceutiques.

Abstract : Did the geological environment accumulate and conserve molecules related to the biomineralization of carbonates? If so, did these molecules retain their biological activity? Sedimentology shows that Provence owned during Cretaceous, 120 million years ago, a tropical shore surrounded by a carbonated platform where biolimestones of high purity accumulated such as in Orgon. In this site, limestone trapped the organic molecules during diagenesis as inclusions in rudist’s calcitic shell (major producers of carbonates) through a process of dissolution / recrystallization centrifuge. Several protocols were tested to extract these molecules. It provides two main types of organo-mineral complex: an extract rather lipidic, obtained mainly with alcohol and another one rather proteinic obtained with water. Their activity was then tested on skin and bone. The dermatological activity was demonstrated on human skin explants maintained in survival. We observed the renewal of the epidermis in 9 days perfectly regulated, the restructuring of the dermo-epidermal junction and, in the dermis, the densification of the collagen network and hyaluronic acid production. On bone, the extracts stimulated the differentiation of the osteoblast lineage cells and also deposits of hydroxyapatite from stem cells cultures. This work has demonstrated that molecules as old as 120 million years are preserved, exploitable and own a series of therapeutic activities on the skin and bone.
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Submitted on : Wednesday, February 1, 2012 - 11:32:16 AM
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Aurore Dissaux. Etude et valorisation des principes actifs de biominéralisation du calcium pour les applications pharmaceutiques.. Sciences agricoles. Université d'Orléans, 2010. Français. ⟨NNT : 2010ORLE2072⟩. ⟨tel-00665124⟩



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