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Manipulation de microparticules exploitant la force de diélectrophorèse : applications dédiées au tri d'espèces biologiques et à l'assemblage de nano objets

Sven Salomon 1
1 LAAS-NBS - Équipe NanoBioSystèmes
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : Manipulating dispersed microparticles is a challenging task and can be achieved using dielectrophoresis. The dielectrophoretic force is induced by non uniform electric fields which polarize the dispersed particles. The particles are consequently attracted towards high electric field gradient areas or repelled. The research work has been focused on bioparticle sorting and nanowire/nanotube assembly. We developed a novel dielectrophoretic continuous flow sorter using planar micro electrodes coupled to a channel constriction. This design enables a high particle sorting efficiency at low voltages while relying on a simple fabrication and integration process. We numerically simulated the AC electrokinetic effects and the fluid behavior to predict particle trajectories. Simulation results are in accordance with experimental data: 10 and 5 µm polystyrene beads were continuously sorted with 2% errors at flow speeds of 100 µm/s. To demonstrate the interest of our device for cell sorting, we also sorted dead and living yeast cells according to their different dielectric properties. Living cell concentration was enriched by a factor of 4 versus dead cell concentration after passing the sorting device. We also developed a novel nanowire assembly process which relies on the coupling of DEP driven assembly and capillary assembly. Using this process we were able to assemble oriented nano objects over several cm2 with high accuracy. This integration process allows performing a statistical characterization of the assembled nanomaterials. This research work shows that dielectrophoretic forces can be used to assemble and sort microparticles depending on the device configuration.
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  • HAL Id : tel-00662858, version 1

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Sven Salomon. Manipulation de microparticules exploitant la force de diélectrophorèse : applications dédiées au tri d'espèces biologiques et à l'assemblage de nano objets. Micro et nanotechnologies/Microélectronique. Université Paul Sabatier - Toulouse III, 2011. Français. ⟨tel-00662858⟩

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