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Etude de la biosynthese du nad chez les plantes : conséquences physiologiques de sa manipulation chez Arabidopsis thaliana

Abstract : Plant development and functions are underpinned by redox reactions which depend on cofactors such as pyridine nucleotides as nicotinamide adenine dinucleotide (NAD). Beside its redox properties, NAD has recently been implicated in cellular signalling. An inducible system based on Escherichia coli quinolinate phosphoribosyltransferase (QPT) overproduction in transgenic Arabidopsis thaliana was set up as a convenient experimental technique to raise NAD content. This build-up highlights the involvement of NAD in plant-pathogen specific defense mechanisms. Furthermore, manipulating endogenous Arabidopsis thaliana QPT levels was used to deregulate NAD production. Such an approach points out the critical role of NAD in C/N interactions by shaking up nitrogen assimilation upon photorespiratory conditions. These results pave the way for a new understanding of signalling mechanisms involving NAD in plants major metabolic functions.
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Pierre Pétriacq. Etude de la biosynthese du nad chez les plantes : conséquences physiologiques de sa manipulation chez Arabidopsis thaliana. Sciences agricoles. Université Paris Sud - Paris XI, 2011. Français. ⟨NNT : 2011PA112188⟩. ⟨tel-00661699⟩

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