Etude de deux lignées de souris sélectionnées pour leur différence de réponse à la méthionine sulfoximine

Abstract : Certain forms of epilepsies still require understanding their mechanisms to be treated. The model of epileptic animals developed by the Laboratory of Neurobiology is constituted by the lines MSO-Fast and MSO-Slow, convulsing respectively quickly or late after the injection of methionine sulfoximine (MSO). This work aims at analyzing and at comparing these two lines to better understand mechanisms contributing to the appearance of the crises induced by MSO. Both lines were characterized at first by their answers to MSO. The implication of the glutamatergic neurotransmission was then revealed by the administration of convulsants and anticonvulsants as well as by a study of the anxiety and the faculty of learning. The study of the glutamine synthetase, the key-enzyme of the the neurotransmitter glutamate recycling, revealed a lower activity of this enzyme for the line MSO-Fast. The measure of the variation of astrocyte glycogen indicates that the glucidic metabolism, connected to the glutamatergic pathway, participates to the difference of sensibility between both lines. The study of the glycoproteins revealed a modification of N-glycosylation linked to a convulsante dose of MSO. The concentrations of 13 molecules belonging to the family of monoamines were measured by HPLC. This study demonstrates on one hand the strong decrease of tryptophane and tyrosine after injection of MSO and on the other hand the implication of norepinephrine and serotonine, two main neurotransmitters controlling the quantity of astrocyte glycogen.
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Human health and pathology. Université d'Orléans, 2011. French. <NNT : 2011ORLE2023>


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Arnaud Boissonnet. Etude de deux lignées de souris sélectionnées pour leur différence de réponse à la méthionine sulfoximine. Human health and pathology. Université d'Orléans, 2011. French. <NNT : 2011ORLE2023>. <tel-00661297>

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