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Découverte d'éléments cis-régulateurs impliqués dans l'activation transcriptionnelle du génome zygotique dans l'embryon précoce de Drosophila melanogaster

Abstract : In metazoa, transcription is silent during early embryogenesis. In Drosophila melanogaster, recent studies of the zygotic genome activation (ZGA) highlighted the implication of few molecular actors (Zelda, STAT92E), but general regulatory mechanisms remains to be understood. Applying bioinformatics analyses on different type of high-throughput data, I searched for new cis-regulatory elements involved in ZGA. First, through analysis of transcriptome data, I selected a group of genes activated during ZGA. Analysis of their non-coding sequences highlighted six motifs, of which three correspond to known binding motifs and factors (Zelda, Trl and TTK). Systematic research of these motifs led to the prediction of putative cis-regulatory modules (CRMs) for which I defined a specific chromatin environment analyzing occupancy profiles of relevant transcription (co-) factors and modified histones, as well as profiles of chromatin opening. Altogether, these results allowed me to define a regulatory model of the ZGA and select candidate regions for further experimental validation.
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https://tel.archives-ouvertes.fr/tel-00644865
Contributor : Elodie Darbo <>
Submitted on : Wednesday, December 14, 2011 - 1:26:25 PM
Last modification on : Tuesday, October 20, 2020 - 3:10:22 AM
Long-term archiving on: : Thursday, March 30, 2017 - 8:46:58 PM

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Elodie Darbo. Découverte d'éléments cis-régulateurs impliqués dans l'activation transcriptionnelle du génome zygotique dans l'embryon précoce de Drosophila melanogaster. Bio-Informatique, Biologie Systémique [q-bio.QM]. Université de la Méditerranée - Aix-Marseille II, 2011. Français. ⟨tel-00644865v2⟩

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