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Theses

Nanostructure des fibres de fibrine

Abstract : The formation of a fibrin clot is one of the major processes leading to blood coagulation. It involves the polymerization of fibrinogen monomers into a network of fibrin fibers. This network controls the overall mechanical properties of the clot and serves as a scaffold to promote wound healing. However its structure at scales less than one micron is very poorly characterized. We demonstrated that an analysis of the visible light spectra transmitted through fibrin clots enables the simultaneous determination, in quantitative terms and in conditions near physiological, of several key parameters of this nanostructure, i.e. the radius and the protein content of the fibers. This spectrophotometry technique has shown the extraordinary porous nature of these fibers and how the reaction parameters (fibrinogen and thrombin concentrations, temperature, ionic strength) control their size and their porosity. This method was then used to characterize the respective effects on the structure of different anticoagulant molecules, showing the specific action of enoxaparin compared with unfractionated heparin and pentasaccharide. We built a prototype (spectrophotometer), used at hospital, to study the kinetics of fibrin polymerization, not only in purified system in combination with our X-ray spectra, but also in plasmas of patients with bleeding disorders. Discussions are underway with a pharmaceutical company to integrate this method on diagnostic equipment.
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Submitted on : Wednesday, November 9, 2011 - 11:03:04 AM
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  • HAL Id : tel-00639435, version 1

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Christelle Yeromonahos. Nanostructure des fibres de fibrine. Autre. Université de Grenoble, 2011. Français. ⟨NNT : 2011GRENI046⟩. ⟨tel-00639435⟩

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