Abstract : The first part of this thesis focused on the impact of sulphur content in pig diets on the amount of sulphur present in the excretion. The sulphur content of each ingredient potentially used in diet formulation was analyzed. Retention and excretion of sulphur ingested by animals (pigs) were evaluated by an in-vivo study. Sulphur absorbed by the animals is poorly retained, beyond the nutritional need; it is excreted primarily in the form of sulphates in urine. The dynamics of sulphate in the slurry was evaluated by a simulation of anaerobic storage, they are consumed and converted to hydrogen sulphide, which is either transferred to the gas phase, or stored in the liquid phase. The major route of production is the dissimilatory reduction of sulphates. The second part of this thesis is devoted to the fate of sulphur in the biogas plant where the agricultural production of hydrogen sulphide is dependent mixtures of co-substrates digested. An inventory of the different substrates used by the biogas plant at the farm has been achieved. The total sulphur contents and the methanogenic potential for each of these substrates have been determined and sulphidogenic potential was defined (maximum concentration of hydrogen sulphide from biogas for a given substrate). With this dataset, a ratio C / S has been defined, which allowed to classify the different substrates for their potential to produce hydrogen sulphide. Green algae stranding on beaches, low substrate P / S, were tested on pilot anaerobic digester in co-digestion with pig manure. For green algae, the low potential for biogas production coupled with high levels of hydrogen sulphide from biogas measured with our test greatly limits the value of their use in agricultural anaerobic digestion.