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Analyse de la matière organique et ses propriétés dans l’environnement naturel en spectroscopie de fluorescence 3D traitée par PARAFAC

Abstract : Fluorescence excitation and emission matrices (EEM) are used to characterize natural organic matter (NOM). To make best use of this information, PARAFAC, a trilinear algorithm is employed. After removing Rayleigh and Raman scattering and correction of the inner filter effect, this method allows separating the spectral components present in MEEF.This work presents two studies: the characterization and quantification of NOM according to its origin and calculation of environmental complexation constants of NOM towards copper as ionic form.Spectral components and their relative intensities are calculated by PARAFAC from 1146 samples gathered according to the missions, the medium type, or the salinity level. To study these components, a new spectral representation is proposed in order to highlight their spectral variability. The results show that even when samples spectra of various origins are clustered, the overall spectral overlap and the relative intensities remain almost similar. On the whole spectral range, areas corresponding to humic substances are quite variable, compared to the protein zone.Metal complexation by NOM is analyzed by combination of four tools: metal ions logarithmic addition, MEEF measurement, the PARAFAC dissociation method and the PROSECE modelling algorithm. Fluorescence quenching measurement is not only limited to the modelling of fluorescence intensity but also to the relative intensity of each PARAFAC-dissociated component though surpassing the methods used so far. Finally, the application of this improving technique leads to quantify NOM complexation properties using a two-complexing sites complexation model for each PARAFAC-dissociated component by using the whole fluorescence signal.
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https://tel.archives-ouvertes.fr/tel-00626538
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Submitted on : Monday, September 26, 2011 - 2:32:26 PM
Last modification on : Friday, January 25, 2019 - 3:52:01 PM
Long-term archiving on: : Tuesday, December 27, 2011 - 2:25:51 AM

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  • HAL Id : tel-00626538, version 1

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Huiyu Zhao. Analyse de la matière organique et ses propriétés dans l’environnement naturel en spectroscopie de fluorescence 3D traitée par PARAFAC. Autre. Université de Toulon, 2011. Français. ⟨NNT : 2011TOUL0003⟩. ⟨tel-00626538⟩

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