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Theses

Utilisation de tensioactifs photosensibles pour le photocontrôle de systèmes biomimétiques, macro- et microfluidiques

Abstract : This thesis shows new possibilities of photocontrol of biochemical and physico-chemical systems with photosensitive cationic surfactants. Their diazobenzene group undergoes a reversible trans-cis photoisomerization that induces a polarity modi cation, which has been exploited, in particular for one of these molecules called AzoTAB. First, the mixing of DNA, AzoTAB and a in vitro gene expression medium allows one to increase the production level of a protein after UV illumination. In this case, the photodependant polarity leads to a modi cation of the conformation of DNA and RNA and thus changes speeds of transcription and translation reactions. Through the synthesis of two new AzoTAB analogues with a longer hydrophobic tail, we also studied the optimisation of concentration of molecules which is necessary to observe the DNA conformation transition. Then, the deformation and the rupture of giant unilamellar vesicles (GUV) in an AzoTAB bath has been studied, depending on the external illumination and their composition. The formation of GUV with a narrow size distribution has also been obtained, while performing the controlled deposition of a multilayer of phospholipids on a fl at microstructured substrate. Finally, the photodependant polarity of this surfactant has been exploited to locally modify the energy of an interface. On the one hand, through the photomanipulation of a millimetric oil droplet fl oating on an AzoTAB bath. On the other hand, in a microfl uidic device, where the fragmentation by light of a biphasic laminar flow into microdroplets has been realized, with a spatial selectivity.
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Theses
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https://tel.archives-ouvertes.fr/tel-00610380
Contributor : Antoine Diguet <>
Submitted on : Thursday, July 21, 2011 - 5:19:12 PM
Last modification on : Monday, December 14, 2020 - 9:49:43 AM
Long-term archiving on: : Friday, November 4, 2011 - 3:08:48 PM

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  • HAL Id : tel-00610380, version 1

Citation

Antoine Diguet. Utilisation de tensioactifs photosensibles pour le photocontrôle de systèmes biomimétiques, macro- et microfluidiques. Autre. Université Pierre et Marie Curie - Paris VI, 2011. Français. ⟨tel-00610380⟩

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