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Rôle de la voie de signalisation MAP kinase Mps1 dans la pathogénie fongique et dans le contrôle de l'intégrité de la paroi

Abstract : The cell wall integrity is crucial for the survival of fungi during its development or in reaction in stress conditions. In yeast, the MAP kinase pathway Slt2, and the calcineurin pathway are responsible of the cell wall repair. MPS1, the orthologous of SLT2, in Magnaporthe grisea is essential for the repair of the cell wall and the penetration of the appressorium (Xu, and Al, 1998). In yeast, Slt2 activates the transcription factors Rlm1, Swi4 and Swi6, whereas the calcineurin activates Crz1. In addition, PaIdc1 has a role in the nuclear localization of PaMpk1 (orthologous of Slt2 and Mps1). (Corinne Jamet-Vierny, and Al, 2007), in Podospora anserina. MgIDC1, the gene orthologous of PaIDC1 , likewise, was identified in M. grisea. ScAGS1 (α- glucan synthase), is the principal component of the wall of fungi. CaCAS5, is a transcriptional regulator, controls several genes of the cell wall and ScKnr4 is implied in the regulation of the activity of 1,3-- glucan synthase and the formation of the cell wall These genes was identified at M. grisea and deletion mutants were obtained by the gene replacement in M. grisea. Mutants Guy11ΔKU80Δmps1 and Guy11ΔKU80Δidc1 show a strong reduction of mycelium. Guy11ΔKU80Δmps1, Guy11ΔKU80Δrlm1 and Guy11ΔKU80Δswi4 have a very strong reduction of sporulation. Guy11ΔKU80Δmps1, Guy11ΔKU80Δrlm1 and Guy11ΔKU80Δcrz1 have a strong reduction of pathogenicity. Moreover, mutants Guy11ΔKU80Δmps1 and Guy11ΔKU80Δswi4 are 100% inhibited, in presence of a mixture of Aculéacine and Nikkomycine with low dose (DI20). The results show that in M. grisea, there are two pathways implied in the cell wall integrity. The MAPK pathway Mps1, which controls the tr anscription factors Rlm1, Swi4, Swi6 and the calcineurin pathway, which controls the transcription factor, Crz1. According to this study, SWI4 is implied in the regulation of glucan synthases and chitin synthase genes, when RLM1 is implied only in the regulation of chitin synthase genes. In the calcineurin pathway, CRZ1 controls chitin synthase genes. These studies suggest that the factors of transcription which are controlled by Mps1 and Calcineurin are specialized in the regulation of target genes, specific to the cell wall integrity and cell wall repair.
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Submitted on : Monday, June 27, 2011 - 10:33:34 AM
Last modification on : Wednesday, August 28, 2019 - 1:08:40 AM
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Cemile Ant. Rôle de la voie de signalisation MAP kinase Mps1 dans la pathogénie fongique et dans le contrôle de l'intégrité de la paroi. Biologie végétale. Université Grenoble Alpes, 2011. Français. ⟨NNT : 2011GRENV006⟩. ⟨tel-00603704⟩



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