Nanoparticules fluorescentes à base de Pluronic : application à l'imagerie intravitale de la vascularisation par microscopie à deux photons et au transport de molécules

Abstract : Classic fluorescent dyes are not necessary efficient in two-photon absorption. Their low two-photon absorption efficiency often requires high laser power and important dye concentrations. Therefore, new dyes and other administration strategies need to be developed specifically for intravital two-photon microscopy. In collaboration with chemists, specialized in the synthesis of molecules with a high two-photon absorption cross-section, different dyes have been synthesized. Most of these dyes are hydrophobic and not directly suitable for biological applications. The work presented in this thesis consisted of encapsulating hydrophobic molecules in biocompatible Pluronic block copolymers. In water, Pluronic unimers with hydrophobic and hydrophilic blocks self-assembled in hydrophilic micelles forming a hydrophobic core around the molecules. This strategy has been used already for the transport and delivery of different hydrophobic molecules in living organism. In the present study, this strategy has been transposed to transport ultra sensitive two-photon dyes in the blood plasma for deep vascular imaging in vivo.
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Mathieu Maurin. Nanoparticules fluorescentes à base de Pluronic : application à l'imagerie intravitale de la vascularisation par microscopie à deux photons et au transport de molécules. Autre [cond-mat.other]. Université Grenoble Alpes, 2011. Français. ⟨NNT : 2011GRENY006⟩. ⟨tel-00593468⟩

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