Syndrome métabolique et diabète chez l'Homme. Composition lipidique et oxydation des lipoprotéines de basse densité (LDL) plasmatiques en relation avec l'activation des plaquettes sanguines.

Abstract : Type-2 diabetes and metabolic syndrome are associated with increased oxidative stress and cardiovascular risk. Platelet hyperactivation and dyslipoproteinemia are two major factors favoring atherothrombosis. We have shown that low-density lipoproteins (LDL) from type-2 diabetic patients activate platelets from healthy volunteers. The primary objective of our study was to establish the lipid and lipid peroxide profile of LDL isolated from plasma of volunteers with a metabolic syndrome (SM), of type-1 (DT-1) or type-2 (DT-2) diabetic patients compared to LDL from healthy volunteers (V). The second objective was to explore their role in platelet activation. Only LDL from MS and DT-2 groups have lipid abnormalities (increased triacylglycerols and decreased cholesteryl esters, decreased linoleic acid). LDL from SM, DT-1 and DT-2 groups show oxidative stress as assessed by increased lipid peroxide derivatives (hydroxylated fatty acids, malondialdehyde) and decreased plasmalogens. Compared to platelets incubated with LDL from the V group, platelets incubated with LDL from other groups are activated as shown by the enhancement of the arachidonic acid signalling cascade (p38 MAPK, cytosolic phospholipase A2, thromboxane B2). Thus, in a pre-diabetic state and in type-2 diabetes, LDL undergo lipid and oxidative modifications and activate platelets. Our results show that lipid peroxides in LDL could be the common denominator underlying platelet hyperactivation.
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Theses
Biomolecules. INSA de Lyon, 2010. French


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Submitted on : Wednesday, April 20, 2011 - 11:23:32 AM
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Romain Colas. Syndrome métabolique et diabète chez l'Homme. Composition lipidique et oxydation des lipoprotéines de basse densité (LDL) plasmatiques en relation avec l'activation des plaquettes sanguines.. Biomolecules. INSA de Lyon, 2010. French. <tel-00587355>

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