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Conception et réalisation d'un micro-injecteur matriciel pour la fonctionnalisation des biopuces

Ty Phou 1
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : The aim of this thesis has been to design and fabricate a matrix of ejectors for in-situ synthesis of oligonucleotides on DNA chip. The approach we propose consists using a matrix of ejectors in order to deposit locally droplets of nucleotides on hybridization cells and to synthesize the required oligonucleotide sequences step by step in parallel. The difficulty of this technique lies on the ability of the micro injector head to eject from any ejector independently from each other. The advantages of the method we propose are the low cost and high flexibility of equipment, and the high density of hybridization units that can be fonctionnalised using this approach. Different actuation principles existing in ejection field have been investigated and finally, the thermal actuation has been chosen. This injection working principle is based on the thermal ink jet printing technique. A heater resistor, in close contact with the liquid to be ejected, generates the nucleation of a gas bubble in the fluid. When the pulse current goes through the heater with a sufficient during and power, the bubble growths until to eject a small droplet through the nozzles of the injectors. The particularity of the matrix of ejectors that we have designed, resides in the ability to actuate any ejector independently without thermal interactions between neighboured heaters. With these considerations, simulations have been realized with FEM simulation under ConventorWare software. The final design integrates on a thin dielectric and stress free membrane, polysilicon heater resistors, and SU8 photopolymerised nozzles. The first characterisations of the devices have shown that ejection occurs with pulse durations and tension intensity from 50µs (25V) to 50ms (5V) and that the ejected volume ranges from 0.1pl to 3nl.
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  • HAL Id : tel-00583924, version 1


Ty Phou. Conception et réalisation d'un micro-injecteur matriciel pour la fonctionnalisation des biopuces. Micro et nanotechnologies/Microélectronique. Université Paul Sabatier - Toulouse III, 2003. Français. ⟨tel-00583924⟩



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