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MATRICES FIBRILLAIRES DENSES DE COLLAGÈNE : MATÉRIAUX POUR LA RÉPARATION OSSEUSE ET L'ÉTUDE D'OSTÉOBLASTES EN TROIS DIMENSIONS

Abstract : Bone tissue ensures the continuity and strength of the skeleton, so as an essential role in mineral homeostasis. Many pathologies, associated with bone resection, require materials to fill the defect. This thesis evaluates the bone healing properties of materials made with collagen solutions at medium (5mg/mL) and high (40mg/mL) concentrations. These two scaffolds are structurally described by light and electron microscopy and compared with classicalyl used freeze-dried collagen sponges. In long term in vitro culture, transformed and primary osteoblasts proliferate, present a cuboidal shape and are arranged in monolayer at the surface of the 5mg/mL matrix, instead of a more resting morphology on the 40mg/mL scaffolds and sponges. In presence of inorganic phosphate, both fibrillar matrices are mineralised by the activity of osteoblasts, despite sponges exhibited spontaneous mineralization. When added inside a rat skull defect, collagen matrices enhanced the healing up to 80% of the surface fill by new bone, compared with 40% without treatment. Collagen density modulates the degradation of the scaffolds, but both are colonized by vascular cells and osteprogenitors that synthesise new bone. With combined microscopy techniques, we observed that primary osteoblasts, inside the three dimensional collagen fibrils network during in vitro cultures, follow a morphological transition. Osteoblasts inside the matrix exhibit long cytoplasmic processes and are connected together, and with the surface, by gap junctions and thus could be related to osteocytes. This work enlightens the potential of the collagen dense fibrillar matrices for bone repair as well as ECM models for in vitro studies.
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https://tel.archives-ouvertes.fr/tel-00574654
Contributor : Sylvain Vigier <>
Submitted on : Tuesday, March 8, 2011 - 3:35:10 PM
Last modification on : Tuesday, September 22, 2020 - 3:58:44 AM
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  • HAL Id : tel-00574654, version 1

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Sylvain Vigier. MATRICES FIBRILLAIRES DENSES DE COLLAGÈNE : MATÉRIAUX POUR LA RÉPARATION OSSEUSE ET L'ÉTUDE D'OSTÉOBLASTES EN TROIS DIMENSIONS. Ingénierie biomédicale. Université Pierre et Marie Curie - Paris VI, 2008. Français. ⟨tel-00574654⟩

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