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MAGNETIC RESONANCE IMAGING OF THE CEREBRAL BLOOD VOLUME FOR THE CHARACTERIZATION OF EXPERIMENTAL BRAIN TUMOR NEOVASCULARIZATION

Abstract : CEREBRAL BLOOD VOLUME FRACTION (CBVF) MAPPING BY MAGNETIC RESONANCE IMAGING (MRI) CAN PROVIDE INFORMATION ABOUT THE PROGRESSION OF TUMOR ANGIOGENESIS WITHOUT HARMFUL SIDE-EFFECTS. IN THIS WORK, A NOVEL MRI METHOD FOR IN VIVO CBVF MAPPING IS DEVELOPED: THE RAPID STEADY STATE T1 (RSST1) METHOD. THE METHOD IS BASED ON A TWO-COMPARTMENT MODEL, INTRA- AND EXTRAVASCULAR, WITHOUT WATER EXCHANGE, AND ON THE LONGITUDINAL RELAXIVITY OF INTRAVASCULAR MRI CONTRAST AGENTS (CAS). THIS METHOD HAS BEEN VALIDATED ON HEALTHY WISTAR RATS AT 2.35 T (CBVF: 2 TO 3%) AND ITS SENSITIVITY HAS BEEN EVALUATED IN A HYPERCAPNIA EXPERIMENT (CBVF INCREASE OF 1%/MMHG CO2). IN ORDER TO APPLY THIS METHOD FOR MONITORING DISEASE EVOLUTION OR TREATMENT EFFICACY, CAS ARE EVALUATED THAT DO NOT LEAK ACROSS THE BLOOD BRAIN BARRIER DURING THE MEASURING TIME. TWO EXPERIMENTAL CA, GD-ACX AND SINEREM WERE USED ON TWO RAT GLIOMA MODELS C6 AND RG2. THE CBVF MEASURES IN TUMOR TISSUE OBTAINED WITH GD-ACX ARE CONFIRMED BY A HISTOLOGIC VASCULAR MORPHOMETRIC ANALYSIS. CBVF MAPPING WITH SINEREM NECESSITATES ACQUISITIONS WITH SHORT ECHO TIME. THE MEASURES WERE COMPARED WITH THOSE OBTAINED BY A DELTAR2*-BASED STEADY STATE METHOD USING THE SAME SINEREM INJECTION. IN CASE OF CA EXTRAVASATION, SUCH AS OCCURS IN TUMOR TISSUE WITH CAS APPROVED FOR CLINICAL USE, THE CBVF ALONG WITH THE TRANSFER COEFFICIENT Κ (A MEASURE RELATED TO THE ENDOTHELIAL PERMEABILITY) WERE OBTAINED BY PHARMACOKINETIC TWO-COMPARTMENT ANALYSIS OF DYNAMIC RSST1 ACQUISITIONS. IN CONCLUSION, THE RSST1 METHOD IN CONJUNCTION WITH APPROPRIATE CAS CAN BE USED FOR LONGITUDINAL ANGIOGENESIS STUDIES TO QUANTIFY THE CBVF AND THE VASCULAR PERMEABILITY.
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https://tel.archives-ouvertes.fr/tel-00564020
Contributor : Teodora-Adriana Perles-Barbacaru <>
Submitted on : Monday, February 7, 2011 - 6:05:49 PM
Last modification on : Friday, November 6, 2020 - 4:07:47 AM
Long-term archiving on: : Sunday, May 8, 2011 - 3:49:24 AM

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  • HAL Id : tel-00564020, version 1

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UJF | U836 | UGA

Citation

Teodora-Adriana Perles-Barbacaru. MAGNETIC RESONANCE IMAGING OF THE CEREBRAL BLOOD VOLUME FOR THE CHARACTERIZATION OF EXPERIMENTAL BRAIN TUMOR NEOVASCULARIZATION. Bioengineering. Université Joseph-Fourier - Grenoble I, 2007. English. ⟨tel-00564020⟩

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