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Microscopie non-lineaire polarimetrique dans les milieux moleculaires et biologiques

Abstract : Light-matter interaction in molecular and bio-molecular media can lead to complex processes where optical fields polarizations couple to an assembly of molecular transition dipoles. The manipulation of the optical fields polarization in fluorescence microscopy can in particular give access to fine changes occurring in molecular arrangements. In this PhD thesis we report a method based on a tuneable excitation polarization state complemented by a polarized read-out, applied to polarization-resolved multiphoton fluorescence microscopy. Two-photon fluorescence polarimetry allows to retrieve a quantitative information on the static molecular distribution shape and orientation in different environments such as model lipid membranes, cell membranes, and molecular inclusion compounds that can be strongly heterogeneous. Three-photon fluorescence polarimetry has been furthermore applied in bio-molecular media in order to provide a diagnostics for crystallinity in protein crystals with high sensitivity to their structure and symmetry. The experimental implementation of polarimetric multi-photon microscopy requires to quantify possible polarization distortions originating from the experimental set-up or sample itself, which are thoroughly analyzed.
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Contributor : Alicja Gasecka <>
Submitted on : Friday, January 28, 2011 - 12:01:33 PM
Last modification on : Thursday, January 23, 2020 - 6:22:08 PM
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  • HAL Id : tel-00560415, version 1



Alicja Gasecka. Microscopie non-lineaire polarimetrique dans les milieux moleculaires et biologiques. Physics [physics]. Université Paul Cézanne - Aix-Marseille III, 2010. English. ⟨tel-00560415⟩



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