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La guérison coquillière : un mécanisme de défense de la palourde japonaise Ruditapes philippinarum face au Vibrio tapetis dans le cadre de la maladie de l'anneau brun

Abstract : Since 1987, the Manila clam is prone to a recurrent infection: the Brown Ring Disease (BRD), caused by the bacterium Vibrio tapetis. This infection is characterized by the formation of a brown ring on the inner face of the valves and often provokes the death of the clam after the invasion of its circulatory system by the bacterium. However, some specimens can recover of this infection by mineralizing a new shell layer which covers the brown ring. The aim of this PhD study is to understand and to characterize how the Manila clams are able to resist to the BRD, principally in studying the mechanism of the shell repair. For this, some techniques like the Scanning Electron Microscopy (SEM), the confocale Raman micro-spectrometry, the WDS microprobe, as well as cellular staining, biochemical and the serology were used. The repair layer is composed by aragonite, polyenes, luminescent molecules on Raman spectra and sulphated molecules. The microstructure of the shell repair is often different than the non-BRD-affected inner layer one and shows a great variability. Furthermore, the shell organic matrix of the shell repair is quantitatively and qualitatively different than of the non-BRD-affected inner layer one and shows equally a great variability. Those non-BRD-affected versus repaired differences and those variabilities could be explained by a modification of the secretory regime of the of calcifying mantle cells, as well as a mobilisation of hemocytes beforehand charged in carbonate de calcium insoluble salts at the level of the shell repair zones.
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https://tel.archives-ouvertes.fr/tel-00543317
Contributor : Nolwenn Trinkler <>
Submitted on : Monday, December 6, 2010 - 1:13:00 PM
Last modification on : Wednesday, September 16, 2020 - 9:56:44 AM

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  • HAL Id : tel-00543317, version 1

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Nolwenn Trinkler. La guérison coquillière : un mécanisme de défense de la palourde japonaise Ruditapes philippinarum face au Vibrio tapetis dans le cadre de la maladie de l'anneau brun. Interactions entre organismes. Université de Bretagne occidentale - Brest, 2009. Français. ⟨tel-00543317⟩

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