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. Ayant-démontré-la-possibilité-d, établir une connexion électrique entre l'hydrogénase, le viologène, les nanotubes de carbone multi-parois et l'électrode, nous avons voulu explorer, de manière préliminaire, les potentialités de connexion de cette enzyme via l'immobilisation du viologène sur les MWCNTs par des interactions ?. Pour cela

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C. Nmr, 300 MHz): ? (ppm) = 174

U. Vis, THF): ? max (nm) = 243

C. Nmr, CDCl 3 , 300 MHz): ? (ppm) = 171

U. Vis, THF): ? max (nm) = 243

C. Nmr, CDCl 3 , 300 MHz): ? (ppm) = 151

. Pyrrole-tosylate, 25 g, 3.2 10 -3 mol) was dissolved in a fresh solution of acetone saturated with sodium iodide and the solution was stirred for 3 h at 60 °C. After the mixture has been left overnight at room temperature, 20 mL of CH 2 Cl 2 and 20 mL of water were added. Organic phases were washed with water (3 x 50mL) and dried with sodium sulphate. The solution was filtrated and evaporated to give 1, g of pyrrole-I (XIV) (yield: 99 %)

. Pyrrole-iode, 11 g, 3.2 10 -3 mol) was dissolved in 30 mL of absolute ethanol and a 4-fold molar excess of triethylamine (1.6 mL, 12 10 -3 mol) was added; the solution was stirred overnight at 85 °C. Excess of triethylamine and the solvent were evaporated and the product was dissolved in methanol to realize anion exchange I -?BF 4 -by deposition on the column conditioned with tetrafluoroborhydric acic (1 % V/V in water) After rinsing step of the column with acetonitrile and evaporation of the solvents, g of pyrrole-ammonium (XV) was obtained

C. Nmr, CDCl 3 , 300 MHz): ? (ppm) = 120

. France, coated with two identical Au layers (Ø = 5 mm, thickness = 200 nm) One gold layer was modified with an electrogenerated poly(pyrrole-NTA) film by oxidative electrolysis

. Mc, A Plexiglas cell (V cell = 50 µL) connected to a micro pump (P1, Pharmacia) was used with an applied constant flow rate of 50 µL min ?1 . The inlet and outlet tubes were placed in the same sample for circulating flow

J. Baur, M. Holzinger, C. Gondran, and S. Cosnier, Immobilization of biotinylated biomolecules onto electropolymerized poly(pyrrole-nitrilotriacetic acid)???Cu2+ film, Electrochemistry Communications, vol.12, issue.10, pp.1287-1290, 2010.
DOI : 10.1016/j.elecom.2010.07.001

. Ce-mémoire-est-consacré-au-développement, Cu 2+ a été utilisé pour l'élaboration de capteurs à ADN ou de biocapteurs enzymatiques. Ainsi, il a pu être utilisé avec son partenaire d'affinité, l'histidine, pour l'immobilisation réversible d'oligonucléotides sondes marqués avec des histidines Grâce à une détection sans marquage de la cible, la spectroscopie d'impédance électrochimique, le plus faible seuil de détection de la littérature (10 -15 mol.L -1 ) pour les biocapteurs à détection impédancemétrique a été décrit. Par la suite, un concept totalement novateur et original a été étudié pour l'utilisation ce polymère afin de réaliser l'immobilisation de biomolécules biotinylées. Cette nouvelle interaction NTA/Cu 2+ /biotine a été illustrée par l'ancrage d'enzymes et d'oligonucléotides marqués avec des biotines. Dans un second temps, des architectures tridimensionnelles à base de nanotubes de carbone fonctionnalisés de façon non-covalente ont été considérées. Tout d'abord, la fonctionnalisation multiple de nanotubes de carbone par interactions ?-? avec des pyrènes modifiés en utilisant les trois systèmes affins les plus courants (adamantane/?-cyclodextrine, avidine/biotine et NTA