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Analyse par microfaisceau d'ions. Application à l'étude de la fonction barrière cutanée et à la nanotoxicologie in vitro

Abstract : Since many years, nanosciences are of great interest for researchers and industrialists, with numerous applications in various domains, however, their potential effects on human health have also attracted attention (environmental, industrial and medical exposures). Up to now, it has been very difficult to detect and track metallic oxides and manufactured nanoparticles in biological tissues, most particularly in skin. Thus, it is essential to precise the mechanisms involved in skin barrier function processes face to these exogenous agents and to characterize them in biological models in vitro/in vivo. During my PhD, I had the opportunity to combine quantitative methods of analysis with high resolution imagery techniques (confocal microscopy, transmission electron microscopy and ion beam analysis) in order to characterize: (i) the skin barrier function of an ex vivo pig ear skin model understanding the ion homeostasis behavior face to different stresses, chemical or physical (Collaboration with Hélène Duplan, Pierre Fabre Research Institute), (ii) the impact on viability, accumulation and intracellular distribution of nanoparticles (Titanium Oxides) naked or functionalized with fluorescent dyes (FITC, Rhodamine) (Collaboration with M.H. Delville, Bordeaux Institute of Condense Matter Chemistry). Results show the possibility to define (i) the role of ions in skin barrier function of a biological ex vivo model in native conditions and after exposure to different stresses (ii) in vivo toxicology of manufactured nanoparticles (iii) their future in a biological model of interest (keratinocytes)
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https://tel.archives-ouvertes.fr/tel-00525919
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Submitted on : Wednesday, October 13, 2010 - 11:26:21 AM
Last modification on : Friday, October 23, 2020 - 4:45:21 PM
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M. Simon. Analyse par microfaisceau d'ions. Application à l'étude de la fonction barrière cutanée et à la nanotoxicologie in vitro. Biologie cellulaire. Université Sciences et Technologies - Bordeaux I, 2009. Français. ⟨tel-00525919⟩

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