Influence de la nanostructuration énergétique des substrats dans l'adhésion et la différenciation des cellules neuronales modèles PC12

Abstract : Significant advances have been made in understanding surface adhesion parameters. Several studies recently demonstrated the combined impact of chemical, spatial and mechanical cues of cell culture substrates in controlling cell functions, together with the genetic program of the cell. This study focus on the substratum physical cue that is surface energy, and in particular, on the influence of surface-energy spatial distribution on neuronal cell differentiation. The cell model under consideration is constituted by clonal-line PC12 pheochromocytoma-cells. PC12 cells have the ability to undergo terminal neuronal differentiation, typically when treated with nerve growth factor (NGF). In this study, PC12 cells were seeded on glass surfaces modified by the self-assembly of alkylsiloxanes or of biopolymers such as poly-L-lysine. By changing the structure, ordering and chemical nature of the self-assembled monolayers, the spatial distribution of surface-energy polar and dispersive components is altered. When seeded on well-ordered homogeneous substrates (with CH3, NH2, or OH terminal groups), PC12 cell adhesion is driven by chemical affinity, and only a few cells initiate neurites. Conversely, PC12 cell adhesion is always effective when seeded on highly disordered substrates, whatever couple of chemical groups (CH3/OH or NH2/OH) generates the surface heterogeneities. In addition, high levels of PC12 cell neuritogenesis are observed by less than 48 h of culture, and without NGF treatment. This work demonstrates that surface chemical heterogeneities, that generate nanoscale surface-energy gradients, are critical to biological processes such as nerve regeneration on biomaterials.
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https://tel.archives-ouvertes.fr/tel-00523656
Contributor : Guillaume Lamour <>
Submitted on : Tuesday, October 5, 2010 - 10:01:43 PM
Last modification on : Thursday, April 11, 2019 - 4:02:22 PM
Long-term archiving on : Thursday, January 6, 2011 - 2:37:19 AM

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Guillaume Lamour. Influence de la nanostructuration énergétique des substrats dans l'adhésion et la différenciation des cellules neuronales modèles PC12. Biophysique [physics.bio-ph]. Université Paris-Diderot - Paris VII, 2010. Français. ⟨tel-00523656⟩

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