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Theses

Etude des mécanismes de régulation des canaux potassiques à deux domaines P

Abstract : Potassium channels form the most diverse class of ion channel family. Three families of potassium channels have been identified, including the two P domain potassium channel family. This family is the last one identified and counts 15 members. These channels produce leak currents that play a key role in cellular excitability, and more particularly in seizures, anesthesia, neuroprotection or depression. A detailed study of the mechanisms regulating the channel activity is necessary for a better comprehension of their roles. We studied the protein environment of the TREK1 channel and we showed that a cytosolic protein associated to microtubules, Mtap2, is able to bind TREK1.This association leads to an increase of TREK1 expression at the plasma membrane. This protein is the second identified. Both of them can bind TREK1 simultaneously and have additive effects, placing TREK1 at the center of a protein network. These results show how much the protein environment of channels is important for their activity. We also showed that TWIK1 is localized in a subapical compartment near the plasma membrane. TWIK1 is actually inserted in the membrane then quickly internalized and addressed to the recycling endosomes. The export from this compartment is drived by activation of a Gi coupled receptor. This activation could be the physiological signal leading to an increase of TWIK1 currents at the plasma membrane.
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https://tel.archives-ouvertes.fr/tel-00508672
Contributor : Nadine Loudig <>
Submitted on : Thursday, August 5, 2010 - 9:56:22 AM
Last modification on : Wednesday, October 14, 2020 - 4:22:35 AM
Long-term archiving on: : Thursday, December 1, 2016 - 8:12:41 AM

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  • HAL Id : tel-00508672, version 1

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Magalie Tardy. Etude des mécanismes de régulation des canaux potassiques à deux domaines P. Neurosciences [q-bio.NC]. Université Nice Sophia Antipolis, 2010. Français. ⟨tel-00508672⟩

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