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Electrochemical synchronization of cell spreading on solid surfaces

Abstract : Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE-GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. For 37 from 47 studied cells (80%), successive fluorescent peaks appear, more or less regularly spaced in time, showing an oscillating actin polymerization activity. Remarkably, no maxima appeared before 7 s after the pulse application. Moreover, 29 cells from 37 (79%) had the first peak within 4 seconds interval, between 7.5 s and 11.5 s after the pulse. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.
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Contributor : Marius Socol <>
Submitted on : Wednesday, July 28, 2010 - 6:43:35 PM
Last modification on : Thursday, November 19, 2020 - 12:59:38 PM
Long-term archiving on: : Friday, October 29, 2010 - 10:27:09 AM

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  • HAL Id : tel-00506773, version 1

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Marius Socol. Electrochemical synchronization of cell spreading on solid surfaces. Biological Physics [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2010. English. ⟨tel-00506773⟩

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