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Phylogénie, éléments transposables et évolution de la taille des génomes chez les lupins

Abstract : Genomes are dynamic structures, varying in size and composition, in which retrotransposons play a major role. In this context, our work aims at: 1) clarifying the phylogenetic relationships within genus Lupinus (Fabaceae) using additional nuclear markers (rRNA-ETS and SymRK), 2) assessing the diversity, abundance and role of the retrotransposons Ty1/copia and Ty3/gypsy in Lupinus genome size variation by amplification and in situ hybridization, and 3) sequencing, annotating and comparing a first genomic region available for lupine with homologous regions in other Fabaceae. The obtained phylogenetic framework improves our understanding of the evolutionary history of lupines, and when combined with the exploration of retrotransposons, highlights lineage-specific patterns of genome size variation. Copia and gypsy elements appear to contribute more significantly to genome size differences in Mediterranean lupines than in African lupines, suggesting different mechanisms involved in the genus. This was confirmed at the local scale (SymRK gene region) where these retroelements represent 25% of the analyzed region in Lupinus angustifolius.
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https://tel.archives-ouvertes.fr/tel-00494607
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Submitted on : Wednesday, June 23, 2010 - 4:51:24 PM
Last modification on : Thursday, January 14, 2021 - 11:49:31 AM
Long-term archiving on: : Friday, September 24, 2010 - 5:33:09 PM

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  • HAL Id : tel-00494607, version 1

Citation

Frédéric Mahé. Phylogénie, éléments transposables et évolution de la taille des génomes chez les lupins. Biochimie [q-bio.BM]. Université Rennes 1, 2009. Français. ⟨tel-00494607⟩

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