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Synthesis and Biological Evaluation of Gold Glyconanoparticles: Ligands for Studying Multivalence Effects

Abstract : The implication of carbohydrate interactions in many normal and pathological biological processes (cell communication, adhesion, bacterial and viral invasion, cancer metastasis etc.) has encouraged the development of interdisciplinary research in the fields of glycochemistry and glyobiology. These interactions are typically highly specific, yet low in affinity. Nature overcomes this by presenting multiple copies of both carbohydrate ligands and protein (lectin) receptors. However, the overall interaction observed is significantly enhanced with respect to the sum of the individual interactions. This phenomenon is known as the “cluster glycoside effect”, or “multivalent effect”. Carbohydrate functionalised Gold nanoparticles (glycol-nanoparticles, GNPs) represent a new group of glycoclusters for studying this phenomenon. As well as their relatively simple synthesis, they also offer many physicochemical properties such as tuneable presentation densities, tuneable gold core sizes and size related electronic, magnetic and optical properties. Here we present the synthesis and characterisation of mannose and galactose functionalised GNPs and investigate their interactions with the multivalent proteins (lectins) Con A, BclA and PA-IL, using biophysical techniques, namely surface plasmon resonance (SPR) and isothermal titration microcalorimetry (ITC). It was found that lectin affinities for the GNPs varied significantly with carbohydrate presentation density. As well as being used for interaction studies, GNPs offer interesting potential as novel diagnostic and therapeutic applications in glycobiology, biotechnology and materials science applications.
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Contributor : Michael Reynolds <>
Submitted on : Friday, March 12, 2010 - 10:33:19 AM
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  • HAL Id : tel-00462627, version 1



Michael Reynolds. Synthesis and Biological Evaluation of Gold Glyconanoparticles: Ligands for Studying Multivalence Effects. Chemical Sciences. Université Joseph-Fourier - Grenoble I, 2009. English. ⟨tel-00462627⟩



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