Adhérence de cellules uniques sur supports micro-structurés

Abstract : The cell adhesion is a critical process involved in many fundamental biological phenomena as dierentiation, tissue repair or cell development. This thesis focuses on a study combining experiments and modelization of single cells spreading on micro-fabricated substrates. Experimental results show that the geometrical constraint imposed by the adhesiveness contrast limits the adhesion. Beyond this limitation, a reproducible organization of the actin cytoskeleton of cells spreading on micro-structured materials suggests that simple physical laws govern the process. We have developed a classication method of basic geometrical shapes observed experimentally to obtain robust statistics. Based on the Cellular Potts model, we reproduced experimental results. This energetical model shows that the basic shapes are metastable states used by cells during spreading. The model parameters are linked to relevant biological parameters. We present results that connect the curvature of interfaces to biological parameters. We show that the experimental measurement of this curvature represents the competition between the contractility of stress bers and the elasticity of the actin gel. A correspondence between the physical properties in the model and the biochemical processes that regulate and organize the cellular adhesion is possible.
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Contributor : Benoit Vianay <>
Submitted on : Wednesday, February 10, 2010 - 10:54:59 AM
Last modification on : Wednesday, February 13, 2019 - 10:06:05 AM
Long-term archiving on: Friday, June 18, 2010 - 5:38:02 PM


  • HAL Id : tel-00455350, version 1




Benoit Vianay. Adhérence de cellules uniques sur supports micro-structurés. Biophysique []. Université Joseph-Fourier - Grenoble I, 2009. Français. ⟨tel-00455350⟩



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