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Etude de la résistance à l'anoïkis des cellules de mélanome B16F10 cultivées sur revêtements anti-adhésifs.

Abstract : Anoikis resistance is one of the hallmarks of malignant transformation. Our purpose was to understand why highly metastatic B16F10 melanoma cells are anoikis resistant when cultured on a substratum preventing cell adhesion and inducing cell aggregation. This 3D architecture is more representative for the in vivo situation in cancer. Cellulose and polyHEMA coated Petri-dishes were used as anti-adhesive surfaces and tissue culture polystyrene (tPS) as an adhesive control (2D). The “normal” Swiss 3T3 fibroblasts and MC3T3 pre-osteoblasts were used as anoikis sensitive control. We have identified dysfunctions probably implicated in B16F10 melanoma cell survival. Our data suggest that melanoma cells were resistant to anoikis through activation of RhoA and PI3K/AKT signaling pathway, whereas caspases 3 and 9 were weakly activated. Integrins which mediate intracellular pathways were highly expressed as well as survivin. Inhibition of PI3K/AKT signaling with antagonists or siRNA induced a significant effect on cell proliferation. Besides, fibronectin seems not to be involved in B16F10 cell behaviour according to gene expression (measured by qRT-PCR and targeted by siRNA). Finally, we obtained interesting and promising results about anti-tumorigenic activity of Okra pectin on melanoma cells cultured in 3D architecture.
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Submitted on : Tuesday, February 9, 2010 - 2:36:07 PM
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O. Goundiam. Etude de la résistance à l'anoïkis des cellules de mélanome B16F10 cultivées sur revêtements anti-adhésifs.. Sciences de l'ingénieur [physics]. Université de Technologie de Compiègne, 2009. Français. ⟨tel-00455003⟩

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