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Mise en évidence de l'entrée cellulaire du virus Nipah par macropinocytose : bases moléculaires et inhibition

Abstract : Nipah and Hendra are two zoonotic Paramyxoviruses that have emerged over the last 15 years in South-Eastern Asia and Australia. They are responsible for encephalitis in man with a mortality rate that can exceed 90%. As there are no treatments nor vaccines available these viruses are classified P4 pathogens. By studying the receptor ephrinB2's downregulation, I discovered that Nipah virus uses an endocytic mechanism for entry : the macropinocytosis. The Henipavirus are the only known Paramyxoviruses that use such an entry mechanism. By mimicking the ephrinB2's ligand (EphB4), the viral glycoprotein G causes the retraction of filopodia to engulf the virus and form macropinosomes. I have demonstrated that the entry of these viruses can be blocked in vitro by the use of drugs that inhibit macropinocytosis. As some of these inhibitors are already used in human medicine, this could pave the way for a low-cost treatment of these dangerous pathogens to be developed.
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https://tel.archives-ouvertes.fr/tel-00448339
Contributor : Olivier Pernet <>
Submitted on : Monday, January 18, 2010 - 5:02:55 PM
Last modification on : Wednesday, November 20, 2019 - 7:44:10 AM

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Olivier Pernet. Mise en évidence de l'entrée cellulaire du virus Nipah par macropinocytose : bases moléculaires et inhibition. Sciences du Vivant [q-bio]. Ecole normale supérieure de lyon - ENS LYON, 2009. Français. ⟨tel-00448339⟩

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