P. Tabeling, Introduction à la Microfluidique, Edition Belin. 4. All the People of the World, Wikipédia, in Wikipédia: World, 2003.

S. Johannes, Aemamentarium chirurgicum XLIII tabulis 1655

B. Alberts, Biologie moléculaire de la cellule, 1995.

A. Abbasauteur, Les bases de l'immunologie fondamentale et clinique, Electrokinetic Flow Diagnostics. Micro-and Nano-Scale Diagnostic Techniques, 2003.

A. Bruce, L'essentiel de la biologie cellulaire 2e édition Médecine-Science, 2000.

E. Gilboa, The promise of cancer vaccines, Nature Reviews Cancer, issue.4, pp.401-411, 2004.

P. Futreal, A census of human cancer genes, Nature Reviews Cancer, vol.4, issue.3, pp.177-83, 2004.
DOI : 10.1038/nrc1299

W. Marion and W. R. Vi, Etude des procédés de fabrication de microdispositifs électromagnétiques sur supports souples pour l'imagerie médicale (IRM) et le contrôle non destructif des matériaux Richard Feynman, Leighton, and Sands, Le cours de physique de Feynman, Electromagnétisme 1 The Hallmarks of Cancer Drew PARDOLL, Does the immune system see tumors as foreign or self? Annual review of immunology, ed. InterEditions. Cell, vol.16, issue.21, pp.57-70, 1979.

G. Fuhr, W. N. Arnold, and R. Hagedorn, Levitation, holding, and rotation of cells within traps made by high-frequency fields, Biochimica et Biophysica Acta (BBA) - Biomembranes, vol.1108, issue.2, pp.1108-215, 1992.
DOI : 10.1016/0005-2736(92)90028-K

L. Saias, A. Saliba, and J. Viovy, Microfluidic magnetic cell sorting system for cancer diagnosis, in µFLU08, 2008.

H. Tanaka, K. S. , T. Hayashi, and S. Shu, Therapeutic immune response induced by electrofusion of dendritic and tumor cells, Cellular Immunology, vol.220, issue.1, pp.1-12, 2002.
DOI : 10.1016/S0008-8749(03)00009-1

R. Doll, Mortality in relation to smoking: 50 years' observations on male British doctors, BMJ, vol.328, issue.7455, pp.1519-182, 2004.
DOI : 10.1136/bmj.38142.554479.AE

J. Schaper and A. , Development of a Technology for Arranged Electrofusion of Mammalian Cells: Applicability in Breast Cancer Immunotherapy Adoptive cell transfer: a clinical path to effective cancer immunotherapy, Nature Reviews Cancer, issue.8, pp.299-308, 2007.

J. Voldman, ELECTRICAL FORCES FOR MICROSCALE CELL MANIPULATION, Annual Review of Biomedical Engineering, vol.8, issue.1, 2006.
DOI : 10.1146/annurev.bioeng.8.061505.095739

T. Akagi and T. Ichiki, Cell electrophoresis on a chip: what can we know from the changes in electrophoretic mobility?, Analytical and Bioanalytical Chemistry, vol.1184, issue.7, pp.2433-2441, 2008.
DOI : 10.1007/s00216-008-2203-9

J. Kao, Superior efficacy of dendritic cell-tumor fusion vaccine compared with tumor lysate-pulsed dendritic cell vaccine in colon cancer, Immunology Letters, vol.101, issue.2, pp.154-163, 2005.
DOI : 10.1016/j.imlet.2005.05.006

P. Abgrall, M. Polymeres, . Les, I. U. Sur-puces, . Physique et al., A new microsystem for automated electrorotation measurements using laser tweezers, BIOCHIMICA ET BIOPHYSICA ACTA- BIOENERGETICS, issue.1, pp.1459-218, 2000.

M. Righini, Parallel and selective trapping in a patterned plasmonic landscape, Nature Physics, issue.3, 2007.

W. H. Edwin, O. Jager, I. Inganäs, and . Lundström, Microrobots for Micrometer-Size Objects in Aqueous Media: Potential Tools for Single-Cell Manipulation, Science, p.288, 2000.

H. Joensuu, P. , P. Bono, and T. Alanko, Adjuvant Docetaxel or Vinorelbine with or without Trastuzumab for Breast Cancer, New England Journal of Medicine, vol.354, issue.8, pp.354-809, 2006.
DOI : 10.1056/NEJMoa053028

K. C. Trevor, Y. ;. Ruiz, . Akporiaye, . Hersh, . Landais et al., Generation of dendritic cell?tumor cell hybrids by electrofusion for clinical vaccine application, Cancer Immunology, Immunotherapy, vol.53, issue.8, pp.53-705, 2004.
DOI : 10.1007/s00262-004-0512-1

T. G. Leonga, In vitro dendritic cell-induced T cell responses to B cell chronic lymphocytic leukaemia enhanced by IL-15 and dendritic cell?B-CLL electrofusion hybrids Eliciting cytotoxic T lymphocytes against acute myeloid leukemia-derived antigens: evaluation of dendritic cell?leukemia cell hybrids and other antigen-loading strategies for dendritic cell-based vaccination, Tetherless thermobiochemically actuated microgrippers. PNAS Clin Exp Immunol Cancer Immunol Immunother, vol.106, issue.396, pp.703-708, 2002.

Y. Tixier-mita and H. Mita, A simple, robust and controllable nano-structures fabrication technique using standard silicon wafers IFN gamma and lymphocytes prevent primary tumour development and shape tumour immunogenicity, Nature, issue.410, pp.1107-1111, 2001.

D. Falconneta, Surface engineering approaches to micropattern surfaces for cell-based assays, Biomaterials, vol.27, issue.16, pp.3044-3063, 2006.
DOI : 10.1016/j.biomaterials.2005.12.024

K. Shortman and S. H. Naik, Steady-state and inflammatory dendritic-cell development The significance of unstable chromosomes in colorectal cancer, Nature Reviews Immunology Nature Reviews Cancer, issue.7 3, pp.19-30, 2003.

R. Fodde, R. Smits, and H. Clevers, APC, Signal transduction and genetic instability in colorectal cancer, Nature Reviews Cancer, vol.1, issue.1, pp.55-67, 2001.
DOI : 10.1038/35094067

S. Petra, A. Dittrich, and . Manz, Lab-on-a-chip: microfluidics in drug discovery, Nature, pp.5-47, 2006.

W. Tan and S. Takeuchi, A trap-and-release integrated microfluidic system for dynamic microarray applicati, pp.1146-1151, 2006.

S. Dertinger, Generation of Gradients Having Complex Shapes Using Microfluidic Networks, Analytical Chemistry, vol.73, issue.6, pp.1240-1246, 2001.
DOI : 10.1021/ac001132d

L. Vladimir and . Sukhorukov, A biophysical approach to the optimisation of dendritic-tumour cell electrofusion, Biochemical and Biophysical Research Communications, vol.346, pp.829-839, 2006.

M. Frenea, Positioning living cells on a high-density electrode array by negative dielectrophoresis, MATERIALS, vol.23, issue.5, pp.597-603, 2003.
URL : https://hal.archives-ouvertes.fr/hal-00739225

P. J. Leea, P. J. Hunga, and L. P. Lee, Microfluidic application-specific integrated device for monitoring direct cell-cell communication via gap junctions between individual cell pairs What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues, APPLIED PHYSICS LETTERS Mol Biotechnol, issue.41, pp.286-295, 2005.

B. Graß, Micro-structured analytical instrumentation for the analysis of liquids, Spectrochimica Acta Part B: Atomic Spectroscopy, vol.57, issue.10, pp.1575-1583, 2002.
DOI : 10.1016/S0584-8547(02)00102-7

W. Coley, Treatment of inoperable malignant tumors with toxins of erysipelas and the bacillus prodigiosus. Tram Am Surg Assoc, pp.183-212, 1894.

L. Gross, Intradermal immunization of C3H mice against a sarcoma that originated in an animal of the same line, Cancer Res, issue.3, pp.326-333, 1943.

E. Foley, Antigenic properties of methylcholanthrene-induced tumors in mice of the strain of origin, Cancer Res, vol.13, pp.835-837, 1953.

L. Thomas, Discussion of Cellular and Humoral Aspects of the Hypersensitive States The concept of immunological surveillance, Prog Exp Tumor Res, issue.13, pp.529-532, 1959.

P. James, J. Goedert, and M. , Spectrum of AIDS-associated malignant disorders. The Lancet, pp.351-1833, 1998.

L. Odile and G. Loïc, Épidémiologie des tumeurs malignes associées à l'infection par le VIH, Bull Cancer, vol.90, issue.5, pp.387-92, 2003.

E. Engels, Trends in cancer risk among people with AIDS in the United States 1980???2002, AIDS, vol.20, issue.12, pp.1645-54, 1980.
DOI : 10.1097/01.aids.0000238411.75324.59

G. Barbaro and G. Barbarini, HIV infection and cancer in the era of highly active antiretroviral therapy (Review), Oncology Reports, vol.17, issue.5, pp.1121-1126, 2007.
DOI : 10.3892/or.17.5.1121

S. Roithmaier and M. , Incidence of Malignancies in Heart and/or Lung Transplant Recipients: A Single-Institution Experience The Journal of Heart and Lung Transplantation, pp.26-845, 2007.

D. H. Kaplan, Demonstration of an interferon ??-dependent tumor surveillance system in immunocompetent mice, Proceedings of the National Academy of Sciences, vol.95, issue.13, pp.7556-7561, 1998.
DOI : 10.1073/pnas.95.13.7556

L. Lewis and . Lanier, A renaissance for the tumor immunosurveillance hypothesis, Nature Medecine, vol.7, pp.1178-1180, 2001.

W. Zou, Regulatory T cells, tumour immunity and immunotherapy, Nature Reviews Immunology, vol.306, issue.4, pp.295-307, 2006.
DOI : 10.1038/nri1806

S. Gross and P. Walden, Immunosuppressive mechanisms in human tumors: Why we still cannot cure cancer, Immunology Letters, vol.116, issue.1, pp.7-14, 2008.
DOI : 10.1016/j.imlet.2007.11.012

D. Gabrilovich, Vascular endothelial growth factor inhibits the development of dendritic cells and dramatically affects the differentiation of multiple hematopoietic lineages in vivo, Blood, issue.11, pp.92-4150, 1998.

W. Chen, Conversion of peripheral CD4(+)CD25(-) naive T cells to CD4(+)CD25(+) regulatory T cells by TGF-beta induction of transcription factor Foxp3, JOURNAL OF EXPERIMENTAL MEDICINE, issue.12, pp.198-1875, 2003.

M. Ralph, J. Steinman, and . Banchereau, Taking dendritic cells into medicine, Nature, vol.449, pp.419-426, 2007.

J. Banchereau and A. Palucka, Dendritic cells as therapeutic vaccines against cancer, Nature Reviews Immunology, vol.10, issue.4, pp.296-306, 2005.
DOI : 10.1007/s00262-003-0429-0

C. Caux, GM-CSF and TNF-?? cooperate in the generation of dendritic Langerhans cells, Nature, vol.360, issue.6401, pp.258-261, 1992.
DOI : 10.1038/360258a0

N. Romani, Proliferating dendritic cell progenitors in human blood, Journal of Experimental Medicine, vol.180, issue.1, pp.83-93, 1994.
DOI : 10.1084/jem.180.1.83

X. Préville, Eradication of Established Tumors by Vaccination With Recombinant Bordetella pertussis Adenylate Cyclase Carrying the Human Papillomavirus 16 E7 Oncoprotein, Cancer Research, vol.65, pp.641-649, 2005.

T. D. De-gruijl, Prolonged Maturation and Enhanced Transduction of Dendritic Cells Migrated from Human Skin Explants After In Situ Delivery of CD40-Targeted Adenoviral Vectors, The Journal of Immunology, vol.169, issue.9, pp.5322-5331, 2002.
DOI : 10.4049/jimmunol.169.9.5322

D. M. Ashley, Bone Marrow???generated Dendritic Cells Pulsed with Tumor Extracts or Tumor RNA Induce Antitumor Immunity against Central Nervous System Tumors, The Journal of Experimental Medicine, vol.5, issue.7, p.186, 1997.
DOI : 10.1097/00002371-199310000-00005

F. I. Viggo and . Van-tendeloo, Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells, Blood, issue.1, pp.98-147, 2001.

D. Xia, T. Moyana, and J. Xiang, Combinational adenovirus-mediated gene therapy and dendritic cell vaccine in combating well-established tumors, Cell Research, vol.171, issue.3, pp.241-259, 2006.
DOI : 10.1084/jem.192.1.145

J. W. Wojcieszyn, Studies on the mechanism of polyethylene glycol-mediated cell fusion using fluorescent membrane and cytoplasmic probes, The Journal of Cell Biology, vol.96, issue.1, pp.151-159, 1983.
DOI : 10.1083/jcb.96.1.151

G. Köhler and C. Milstein, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature, vol.3, issue.5517, pp.495-497, 1975.
DOI : 10.1038/256495a0

T. Scott-taylor, Human tumour and dendritic cell hybrids generated by electrofusion: potential for cancer vaccines, Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, vol.1500, issue.3, pp.1500-265, 2000.
DOI : 10.1016/S0925-4439(99)00108-8

G. Stuhler, U. Trefzer, and W. P. , Hybrid cell vaccination in cancer immunotherapy -Recruitment and activation of T cell help for induction of anti tumour cytotoxic T cells, ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY, 1998.

M. Parkhurst, Hybrids of Dendritic Cells and Tumor Cells Generated by Electrofusion Simultaneously Present Immunodominant Epitopes from Multiple Human Tumor-Associated Antigens in the Context of MHC Class I and Class II Molecules, The Journal of Immunology, vol.170, issue.10, pp.5317-5325, 2003.
DOI : 10.4049/jimmunol.170.10.5317

Y. Takashi, Dendritic cell-tumor cell hybrids enhance the induction of cytotoxic T lymphocytes against murine colon cancer: A comparative analysis of antigen loading methods for the vaccination of immunotherapeutic dendritic cells, Oncology Reports, issue.6, pp.16-1317, 2006.

T. Yasuda, Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma, Cancer Immunology, Immunotherapy, vol.101, issue.7, pp.1025-1036, 2007.
DOI : 10.1007/s00262-006-0252-5

. Kikuchi-tetsuro, Results of a phase 1 clinical trial of vaccination of glioma patients with fusion of dendritic and glioma cells. Cancer immunology and immunotherapy, pp.50-337, 2001.

D. Avigan, Fusion Cell Vaccination of Patients with Metastatic Breast and Renal Cancer Induces Immunological and Clinical Responses, Clinical Cancer Research, vol.10, issue.14, pp.4699-4708, 2004.
DOI : 10.1158/1078-0432.CCR-04-0347

Y. Wei, Dendritoma vaccination combined with low dose interleukin-2 in metastatic melanoma patients induced immunological and clinical responses, International Journal of Oncology, vol.28, issue.3, pp.585-593, 2006.
DOI : 10.3892/ijo.28.3.585

M. Karen, ;. Joseph, J. Steven, ;. Thomas, E. et al., Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study, Oncology Reports, issue.3, p.18, 2007.

A. Märten, Allogeneic Dendritic Cells Fused with Tumor Cells: Preclinical Results and Outcome of a Clinical Phase I/II Trial in Patients with Metastatic Renal Cell Carcinoma, Human Gene Therapy, vol.14, issue.5, pp.20-483, 2003.
DOI : 10.1089/104303403321467243

J. Alexandre and M. Barbuto, Dendritic cell?tumor cell hybrid vaccination for metastatic cancer, Cancer Immunol Immunother, vol.53, issue.12, pp.1111-1118, 2004.

. Trefzer-uwe, Tumour-dendritic hybrid cell vaccination for the treatment of patients with malignant melanoma: immunological effects and clinical results, Vaccine, vol.23, issue.17-18, pp.17-18, 2005.
DOI : 10.1016/j.vaccine.2005.01.081

I. Jolanda and M. De-vries, Effective Migration of Antigen-pulsed Dendritic Cells to Lymph Nodes in Melanoma Patients Is Determined by Their Maturation State, Cancer Res, p.63, 2003.

E. Gottfried, Characterization of cells prepared by dendritic cell-tumor cell fusion, Cancer Immun, issue.2, 2002.

R. P. Feynman, Plenty of Room at the Bottom. 1959 [cited; Available from

T. Stephen and J. Angell, A Gas Chromatographic Air Analyzer Fabricated, IEEE Transactions On Electron Devices, p.12, 1979.

J. El-ali, P. K. Sorger, and K. F. Jensen, Cells on chips, Nature, vol.4, issue.7101, p.442, 2006.
DOI : 10.1038/nature05063

D. Laser and J. Santiago, A review of micropumps, Journal of Micromechanics and Microengineering, vol.14, issue.6, p.14, 2004.
DOI : 10.1088/0960-1317/14/6/R01

P. Woias, Micropumps???past, progress and future prospects, Sensors and Actuators B: Chemical, vol.105, issue.1, pp.28-38, 2005.
DOI : 10.1016/S0925-4005(04)00108-X

A. Ashkin, Optical trapping and manipulation of neutral particles using lasers, pp.4853-4860, 1997.

G. David and . Grier, A revolution in optical manipulation, Nature, p.424, 2003.

U. Bora, L. Chugh, and P. Nahar, Covalent immobilization of proteins onto photoactivated polystyrene microtiter plates for enzyme-linked immunosorbent assay procedures, Journal of Immunological Methods, vol.268, issue.2, pp.171-177, 2002.
DOI : 10.1016/S0022-1759(02)00212-0

Z. Wang and G. Jin, Covalent immobilization of proteins for the biosensor based on imaging ellipsometry, Journal of Immunological Methods, vol.285, issue.2, pp.237-243, 2004.
DOI : 10.1016/j.jim.2003.12.002

B. L. Pioufle, Living cells captured on a bio-microsystem devoted to DNA injection, Materials Science and Engineering: C, vol.12, issue.1-2, pp.77-81, 2000.
DOI : 10.1016/S0928-4931(00)00162-4

URL : https://hal.archives-ouvertes.fr/hal-00739228

J. Schaper, H. R. Bohnenkamp, and T. Noll, New Electrofusion Devices for the Improved Generation of Dendritic Cell-tumour Cell Hybrids, in Cell Technology for Cell Products, pp.207-216, 2007.

A. M. Martin and . Gijs, Magnetic bead handling on-chip: new opportunities for analytical applications, Microfluid Nanofluid, pp.22-40, 2004.

R. Fulcrand, Development of flexible polymer lab on chip integrating microelectromagnets for microbeads manipulation, in µFLU08, 2008.

M. Frenea-robin, Contactless diamagnetic trapping of living cells onto a micromagnet array, in EMBC, 2008.

R. F. Probstein, Physicochemical Hydrodynamics: An Introduction. 2 ed, 1994.
DOI : 10.1002/0471725137

H. Nagata, M. T. , K. Hirano, and Y. Baba, High-speed separation of proteins by microchip electrophoresis using a polyethylene glycol-coated plastic chip with a sodium dodecyl sulfate-linear polyacrylamide solution, ELECTROPHORESIS, vol.3, issue.14, pp.26-2687, 2005.
DOI : 10.1002/elps.200410337

N. Kaji, Separation of Long DNA Molecules by Quartz Nanopillar Chips under a Direct Current Electric Field Anal, Chem, vol.76, issue.1, pp.15-22, 2004.

T. Mari, Nanospheres for DNA separation chips, Nature biotechnology, vol.22, issue.3, pp.337-340, 2004.

A. Y. Fu, A microfabricated fluorescence-activated cell sorter, NATURE BIOTECHNOLOGY, p.17, 1999.

M. Evander, Noninvasive Acoustic Cell Trapping in a Microfluidic Perfusion System for Online Bioassays, Analytical Chemistry, vol.79, issue.7, pp.2984-2991, 2007.
DOI : 10.1021/ac061576v

C. Bottier, Kinesin-Based transportation and electrofusion of lipid vesicles, in µTAS, 2008.

J. Mehrishi and J. Bauer, Electrophoresis of cells and the biological relevance of surface charge, ELECTROPHORESIS, vol.23, issue.13, pp.1984-1994, 2002.
DOI : 10.1002/1522-2683(200207)23:13<1984::AID-ELPS1984>3.0.CO;2-U

T. B. Jones, Electromechanics of Particles, 1995.
DOI : 10.1017/CBO9780511574498

E. Muth, Ueber die Erscheinung der Perlschnurkettenbildung von Emulsionspartikelchen unter Einwirkung eines Weshselfelds, p.41, 1927.

P. Liebesny, Athermic short wave therapy, Arch. Phys. Ther, issue.736, p.19, 1939.

H. Pohl, The Motion and Precipitation of Suspensoids in Divergent Electric Fields, Journal of Applied Physics, vol.22, issue.7, pp.869-871, 1951.
DOI : 10.1063/1.1700065

A. Castellanos, Electrohydrodynamics and dielectrophoresis in microsystems: scaling laws, Journal of Physics D: Applied Physics, vol.36, issue.20, pp.2584-2597, 2003.
DOI : 10.1088/0022-3727/36/20/023

T. Müller, A 3-D microelectrode system for handling and caging single cells and particles, Biosensors and Bioelectronics, vol.14, issue.3, pp.247-256, 1999.
DOI : 10.1016/S0956-5663(99)00006-8

F. F. Becker, Separation of human breast cancer cells from blood by differential dielectric affinity., Proceedings of the National Academy of Sciences, vol.92, issue.3, pp.860-864, 1995.
DOI : 10.1073/pnas.92.3.860

H. Morgan, M. P. Hughes, and N. G. Green, Separation of Submicron Bioparticles by Dielectrophoresis, Biophysical Journal, vol.77, issue.1, pp.516-525, 1999.
DOI : 10.1016/S0006-3495(99)76908-0

J. Voldman, Holding Forces of Single-Particle Dielectrophoretic Traps, Biophysical Journal, vol.80, issue.1, pp.531-541, 2001.
DOI : 10.1016/S0006-3495(01)76035-3

J. Voldman, A Microfabrication-Based Dynamic Array Cytometer, Analytical Chemistry, vol.74, issue.16, pp.74-3984, 2002.
DOI : 10.1021/ac0256235

A. Rosenthal and J. Voldman, Dielectrophoretic Traps for Single-Particle Patterning, Biophysical Journal, vol.88, issue.3, pp.2193-2205, 2005.
DOI : 10.1529/biophysj.104.049684

URL : http://doi.org/10.1529/biophysj.104.049684

M. Brian, J. Taff, and . Voldman, A Scalable Addressable Positive-Dielectrophoretic Cell-Sorting Array, ANALYTICAL CHEMISTRY, vol.77, pp.7976-7983, 2005.

D. R. Albrecht, Photo- and electropatterning of hydrogel-encapsulated living cell arrays, Lab on a Chip, vol.5, issue.1, pp.111-118, 2004.
DOI : 10.1039/b406953f

H. Blanca and . Lapizco-encinas, Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators, ANALYTICAL CHEMISTRY, vol.76, pp.1571-1579, 2004.

Y. Kang, DC-Dielectrophoretic separation of biological cells by size, Biomedical Microdevices, vol.86, issue.2, pp.243-249, 2008.
DOI : 10.1007/s10544-007-9130-y

I. Barbulovic-nad, DC-dielectrophoretic separation of microparticles using an oil droplet obstacle, Lab Chip, vol.70, issue.4, pp.274-279, 2006.
DOI : 10.1039/B513183A

S. Theodore, R. A. Jardetzky, and . Lamb, A class act, Nature, p.427, 2004.

C. Barrau, J. T. , and B. Gabriel, Osmotically induced membrane tension facilitates the triggering of living cell electropermeabilization, Bioelectrochemistry, vol.63, issue.1-2, pp.327-332, 2004.
DOI : 10.1016/j.bioelechem.2003.11.009

J. Teissié and M. Rols, An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization, Biophysical Journal, vol.65, issue.1, pp.409-413, 1993.
DOI : 10.1016/S0006-3495(93)81052-X

M. Lluis and . Mir, Application of Electroporation Gene Therapy: Past, Current, and Future, Methods in Molecular Biology, p.423, 2008.

C. Chen, Membrane electroporation theories: a review, Medical & Biological Engineering & Computing, vol.7, issue.5, pp.5-14, 2006.
DOI : 10.1007/s11517-005-0020-2

J. Teissié, M. Golzio, and M. P. Rols, Mechanisms of cell membrane electropermeabilization: A minireview of our present (lack of ?) knowledge, Biochimica et Biophysica Acta (BBA) - General Subjects, vol.1724, issue.3, pp.1724-270, 2005.
DOI : 10.1016/j.bbagen.2005.05.006

J. Teissie and M. P. Rols, Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, pp.258-266, 1986.

D. Peter-tieleman, Simulation of Pore Formation in Lipid Bilayers by Mechanical Stress and Electric Fields, Journal of the American Chemical Society, vol.125, issue.21, pp.125-6382, 2003.
DOI : 10.1021/ja029504i

R. A. Bockmann, Kinetics, Statistics, and Energetics of Lipid Membrane Electroporation Studied by Molecular Dynamics Simulations, Biophysical Journal, vol.95, issue.4, pp.1837-1850, 2008.
DOI : 10.1529/biophysj.108.129437

M. Tarek, Membrane Electroporation: A Molecular Dynamics Simulation, Biophysical Journal, vol.88, issue.6, pp.4055-4053, 2005.
DOI : 10.1529/biophysj.104.050617

A. Andrey, I. Gurtovenko, and . Vattulainen, Molecular Mechanism for Lipid Flip- Flops, J. Phys. Chem. B, issue.11148, pp.13554-13559, 2007.

W. M. Cees, D. Haest, B. Kamp, and . Deuticke, Transbilayer reorientation of phospholipid probes in the human erythrocyte membrane. Lessons from studies on electroporated and resealed cells, Biochimica et Biophysica Acta, pp.1325-1342, 1997.

U. Pliquett, High electrical field effects on cell membranes, Bioelectrochemistry, vol.70, issue.2, pp.275-282, 2006.
DOI : 10.1016/j.bioelechem.2006.10.004

C. Ramos and J. Teissié, Electrofusion: A biophysical modification of cell membrane and a mechanism in exocytosis, Biochimie, vol.82, issue.5, pp.511-518, 2000.
DOI : 10.1016/S0300-9084(00)00200-5

M. R. Parkhurst, Hybrids of Dendritic Cells and Tumor Cells Generated by Electrofusion Simultaneously Present Immunodominant Epitopes from Multiple Human Tumor-Associated Antigens in the Context of MHC Class I and Class II Molecules, The Journal of Immunology, vol.170, issue.10, pp.5317-5325, 2003.
DOI : 10.4049/jimmunol.170.10.5317

K. T. Trevor, Generation of dendritic cell?tumor cell hybrids by electrofusion for clinical vaccine application, Cancer Immunology, Immunotherapy, vol.53, issue.8, pp.705-714, 2004.
DOI : 10.1007/s00262-004-0512-1

G. Tresset and S. Takeuchi, A Microfluidic Device for Electrofusion of Biological Vesicles, Biomedical Microdevices, vol.6, issue.3, pp.213-218, 2004.
DOI : 10.1023/B:BMMD.0000042050.95246.af

Y. Cao, Study of high-throughput cell electrofusion in a microelectrode-array chip. Microfluid Nanofluid, pp.669-675, 2008.

M. Alison and . Skelley, Microfluidic control of cell pairing and fusion, NATURE METHODS, vol.6, issue.2, 2009.

D. Duffy, Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) Analytical Chemistry, p.70, 1998.

J. Marc and . Madou, Fundamentals of microfabrication. 2 ed, p.723, 2002.

J. , C. Mcdonald, and G. M. Whitesides, Poly(dimethylsiloxane) as a Material for Fabricating Microfluidic Devices, Acc. Chem. Res, vol.35, issue.7, pp.491-499, 2002.

J. Ng-lee, C. Park, and G. M. Whitesides, Solvent Compatibility of Poly(dimethylsiloxane)-Based Microfluidic Devices, Anal. Chem, issue.23, pp.75-6544, 2003.

M. Heckele and S. W. , Review on micro molding of thermoplastic polymers, Journal of Micromechanics and Microengineering, vol.14, issue.3, pp.1-14, 2004.
DOI : 10.1088/0960-1317/14/3/R01

M. Denoual, Vacuum casting to manufacture a plastic biochip for highly parallel cell transfection, Measurement Science and Technology, vol.17, issue.12, pp.3134-3140, 2006.
DOI : 10.1088/0957-0233/17/12/S03

URL : https://hal.archives-ouvertes.fr/hal-00739220

. Mircochem and . Su-8, [cited; Available from

A. Del, C. , and C. Greiner, SU-8: a photoresist for high-aspect-ratio and 3D submicron lithography, J. Micromech. Microeng, vol.17, pp.81-95, 2007.

G. Voskericiana, Biocompatibility and biofouling of MEMS drug delivery devices, Biomaterials, vol.24, issue.11, pp.1959-1967, 2003.
DOI : 10.1016/S0142-9612(02)00565-3

G. Kotzara, Evaluation of MEMS materials of construction for implantable medical devices, Biomaterials, vol.23, issue.13, pp.2737-2750, 2002.
DOI : 10.1016/S0142-9612(02)00007-8

H. Lu, SU8-Based micro neural probe for enhanced chronic in-vivo recording of spike signals from regenerated axons, 5th IEEE Sensors Conference, 2006.

Y. Chuang, A novel fabrication method of embedded micro-channels by using SU-8 thick-film photoresists. Sensors and Actuators A-Physical, pp.64-69, 2003.

B. Alderman, Microfabrication of channels using an embedded mask in negative resist, Journal of Micromechanics and Microengineering, vol.11, issue.6, pp.703-705, 2001.
DOI : 10.1088/0960-1317/11/6/312

G. Louis, Simple and low cost fabrication of embedded microchannels by using a new thick-film photoplastic, TRANSDUCERS 97, 1997.

Z. Ling and K. Lian, In situ fabrication of SU-8 movable parts by using PAG-diluted SU-8 as the sacrificial layer, Microsystem Technologies, vol.14, issue.3-4, pp.253-257, 2007.
DOI : 10.1007/s00542-006-0180-5

S. Metz, Polyimide and SU-8 microfluidic devices manufactured by heatdepolymerizable sacrificial material technique, Lab on a Chip, issue.4, pp.114-120, 2004.

P. Abgrall, A novel fabrication method of flexible and monolithic 3D microfluidic structures using lamination of SU-8 films, Journal of Micromechanics and Microengineering, vol.16, issue.1, pp.113-121, 2006.
DOI : 10.1088/0960-1317/16/1/016

S. Tuomikoski and S. Franssila, Free-standing SU-8 microfluidic chips by adhesive bonding and release etching. Sensors and Actuators A, pp.408-415, 2005.

F. Blanco, Novel three-dimensional embedded SU-8 microchannels fabricated using a low temperature full wafer adhesive bonding, Journal of Micromechanics and Microengineering, vol.14, issue.7, pp.1047-1056, 2004.
DOI : 10.1088/0960-1317/14/7/027

V. Mathet, Centrale de micro-technologie. [cited; Available from

C. Ab and . Multiphysics, [cited; Available from

M. Guillaume, D. Matthieu, and L. Bruno, Réalisation d'un biomicrosystème pour la fusion cellulaire en vue de l'immunothérapie du cancer, in JNRDM, 2007.

. Wieweb-software, Layout Software CleWin. [cited; Available from

H. I. Gmbh, M. laser lithography system, 2009.

. Dvh-research-group and . Az-5214e, Available from: http://groups, 2005.

. Microchem and . Omnicoat, [cited; Available from

R. Engelke, Complete 3D UV microfabrication technology on strongly sloping topography substrates using epoxy photoresist SU-8, Microelectronic Engineering, vol.73, issue.74, pp.73-74, 2004.
DOI : 10.1016/S0167-9317(04)00193-5

J. Voldman, A Dielectrophoresis-based Array Cytometer, in Transducers, 2001.

I. Technic, Technic Immersion Gold. [cited; Available from

K. Ivanovich-popov, S. S. Djoki?, and B. N. Grgur, Fundamental aspects of electrometallurgy, 2002.

P. J. Slikkerveer, P. C. Bouten, and F. C. De-haas, High quality mechanical etching of brittle materials by powder blasting, Sensors and Actuators A: Physical, vol.85, issue.1-3, pp.296-303, 2000.
DOI : 10.1016/S0924-4247(00)00343-5

E. Belloy, The introduction of powder blasting for sensor and microsystem applications, Sensors and Actuators A: Physical, vol.84, issue.3, pp.330-337, 2000.
DOI : 10.1016/S0924-4247(00)00390-3

V. André, De humani corporis fabrica libri septem

P. Gosset, hôpital pour cancérés », communication faite à l'académie de Reims, 1926.

F. Blattner, The Complete Genome Sequence of Escherichia coli K-12, Science, vol.277, issue.5331, pp.1453-74, 1997.
DOI : 10.1126/science.277.5331.1453

M. Adams, The Genome Sequence of Drosophila melanogaster, Science, vol.287, issue.5461, pp.2185-95, 2000.
DOI : 10.1126/science.287.5461.2185

M. Genome-sequencing and . Consortium, Initial sequencing and comparative analysis of the mouse genome, Nature, vol.420, pp.520-62, 2002.

F. Eric, R. , and B. Vogelstein, A genetic model for colorectal cancer tumorigenesis, Cell, vol.61, pp.759-767, 1990.

M. Gérard, D. , J. , and G. , Le drame de l'amiante en France : comprendre, mieux réparer, en tirer des leçons pour l'avenir, 2005.

D. R. and H. Ab, Smoking and carcinoma of the lung, Br. Med. J, issue.2, pp.739-787, 1950.

R. Doll and A. B. Hill, The Mortality of Doctors in Relation to Their Smoking Habits, BMJ, vol.1, issue.4877, pp.1451-1456, 1954.
DOI : 10.1136/bmj.1.4877.1451

S. Boseley, Renowned cancer scientist was paid by chemical firm for 20 years. The Guardian, 2005.

C. Sophie and F. Lemarchand, Cancer du sein : les illusions du dépistage La recherche, p.395, 2006.

B. Fisher, Reanalysis and results after 12 years of follow-up in a randomized clinical trial comparing total mastectomy with lumpectomy with or without irradiation in the treatment of breast cancer The New England Journal of Medecine, pp.1456-1461, 1995.

O. Stephen and R. A. Larson, International Randomized Study of Interferon Versus STI571 (IRIS) 7-Year Follow-up: Sustained Survival, Low Rate of Transformation and Increased Rate of Major Molecular Response (MMR) in Patients (pts) with Newly Diagnosed Chronic Myeloid Leukemia in Chronic Phase (CML-CP) Treated with Imatinib (IM) in 5Oth, 2008.

D. V. Mcallister, Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: Fabrication methods and transport studies, Proceedings of the National Academy of Sciences, vol.100, issue.24, pp.13755-13760, 2003.
DOI : 10.1073/pnas.2331316100